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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-6-24
|
| pubmed:abstractText |
We have developed an ELISA to measure murine autoantibodies to the collagenous region (CLR) of C1q, using the whole human C1q molecule as the solid-phase ligand, in the presence of 1 M NaCl. The assay was validated by testing positive sera from 20 mice using purified mouse C1q, and from 10 mice using purified human C1q-CLR, as the solid-phase ligands. There were highly significant correlations between results obtained with human C1q (whole molecule) and: (i) mouse C1q (rsp = 0.73, P less than 0.001), and (ii) human Clq-CLR alone (rsp = 0.86, P = 0.001). Antibodies to Clq were measured in 53 MRL/lpr, 17 BXSB and 25 NZB/W lupus-prone mice. Median (range) anti-C1q (CLR) antibody levels in MRL/lpr, BXSB, and NZB/W autoimmune mice aged 3 months were 22 (16-66), 21 (17-39) and 19 (15-27) EU, respectively. The median anti-Clq antibody level in MRL/lpr mice aged 5 months was 76 (35-142) EU, significantly higher than that at 3 months (U = 558, P less than 0.0005). Median anti-C1q antibody level in NZB/W mice at 8 months was 37 (13-74) EU and in BXSB mice at 11 months was 62 (31-231) EU, significantly higher than corresponding values at 3 months (U = 326, and U = 4, P less than 0.001, respectively). This is the first demonstration of anti-C1q (CLR) antibodies in NZB/W and BXSB mice. The pathologic significance and the potential utility of these antibodies for monitoring disease in lupus-prone mice are under evaluation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C1q,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C3,
http://linkedlifedata.com/resource/pubmed/chemical/DNA
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0009-9104
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
104
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
241-6
|
| pubmed:dateRevised |
2008-11-20
|
| pubmed:meshHeading |
pubmed-meshheading:8625515-Aging,
pubmed-meshheading:8625515-Animals,
pubmed-meshheading:8625515-Antibodies, Antinuclear,
pubmed-meshheading:8625515-Autoantibodies,
pubmed-meshheading:8625515-Collagen,
pubmed-meshheading:8625515-Complement C1q,
pubmed-meshheading:8625515-Complement C3,
pubmed-meshheading:8625515-DNA,
pubmed-meshheading:8625515-Female,
pubmed-meshheading:8625515-Humans,
pubmed-meshheading:8625515-Lupus Erythematosus, Systemic,
pubmed-meshheading:8625515-Male,
pubmed-meshheading:8625515-Mice,
pubmed-meshheading:8625515-Mice, Inbred C57BL,
pubmed-meshheading:8625515-Mice, Inbred CBA,
pubmed-meshheading:8625515-Mice, Inbred NZB,
pubmed-meshheading:8625515-Mice, Mutant Strains,
pubmed-meshheading:8625515-Osmolar Concentration,
pubmed-meshheading:8625515-Protein Binding,
pubmed-meshheading:8625515-Species Specificity
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice.
|
| pubmed:affiliation |
Rheumatology Unit, RPMS, London, UK.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|