Linked Life Data

8624036

Source:http://linkedlifedata.com/resource/pubmed/id/8624036

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-6-14
pubmed:databankReference
pubmed:abstractText
An approach has been developed for the screening of allelic variation of minisatellite DNA loci that substantially reduces the time and hazards involved. Primers were designed for a minisatellite region isolated from a gadoid fish species (Merlangius merlangus L.), enabling amplification by polymerase chain reaction, so that differences in the number of minisatellite repeat units (allelic variability) were detectable by ethidium bromide fluorescence (over UV light) following separation by agarose gel electrophoresis. This amplifiable minisatellite variable number tandem repeat region, the first non-primate marker of its kind can be used successfully with DNA extracted by a rapid Chelex protocol. From a sample of 97 individuals, 24 alleles were resolved (750-2200 kb) and heterozygosity was estimated at 0.94.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0268-9146
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-51
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
PCR amplification of a polymorphic minisatellite VNTR locus in whiting (Merlangius merlangus L.).
pubmed:affiliation
Department of Zoology, University College Cork, Ireland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't