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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-6-14
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21388,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21751,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21752,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21753,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21754,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21755,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21756,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21757,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21758,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21759,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21760,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21761,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21762,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21763,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21764,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21765,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21766,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21767,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21768,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21769,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21770,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21771,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21772,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21773,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21774,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21775,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21776,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21777,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21778,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U21779
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pubmed:abstractText |
A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6.3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0268-9146
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8624035-Animals,
pubmed-meshheading:8624035-Base Sequence,
pubmed-meshheading:8624035-Cattle,
pubmed-meshheading:8624035-DNA Primers,
pubmed-meshheading:8624035-Dinucleotide Repeats,
pubmed-meshheading:8624035-Female,
pubmed-meshheading:8624035-Genomic Library,
pubmed-meshheading:8624035-Male,
pubmed-meshheading:8624035-Microsatellite Repeats,
pubmed-meshheading:8624035-Molecular Sequence Data,
pubmed-meshheading:8624035-Polymorphism, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
Isolation and characterization of 45 polymorphic microsatellites from the bovine genome.
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pubmed:affiliation |
Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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