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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1996-6-18
|
| pubmed:abstractText |
In several tissues including gastric mucosa, somatostatin displays various biological effects. Five seven-transmembrane-domain somatostatin receptor subtypes (SSTR1-5) have been recently cloned and only SSTR1 has been shown to be present in the human stomach. We used the polymerase chain reaction on reverse transcripts (RT-PCR) to characterize further the SSTR's mRNAs in human and rat gastric mucosae and in the human gastric tumoral cell-line HGTL. The SSTR1-5's mRNAs were found in both human fundic and antral mucosae as well as in the HGT1 cell and rat antrum. The four SSTR2-5's mRNA's but not SSTR1's were detected in the rat fundic mucosa. Furthermore, the use of rat isolated and purified fundic mucosal cells allowed us to localize SSTR2-5 in the parietal cell-enriched fraction, whereas SSTR2 and SSTR5 were the only subtypes found in the endocrine cell-enriched fraction. These results are the first to demonstrate the presence of five SSTR's mRNA subtypes in the stomach.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0024-3205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
58
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1091-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8622562-Animals,
pubmed-meshheading:8622562-Base Sequence,
pubmed-meshheading:8622562-Cloning, Molecular,
pubmed-meshheading:8622562-DNA Primers,
pubmed-meshheading:8622562-Female,
pubmed-meshheading:8622562-Gastric Fundus,
pubmed-meshheading:8622562-Gastric Mucosa,
pubmed-meshheading:8622562-Humans,
pubmed-meshheading:8622562-Molecular Sequence Data,
pubmed-meshheading:8622562-Oligonucleotides, Antisense,
pubmed-meshheading:8622562-Polymerase Chain Reaction,
pubmed-meshheading:8622562-RNA, Messenger,
pubmed-meshheading:8622562-Rats,
pubmed-meshheading:8622562-Rats, Sprague-Dawley,
pubmed-meshheading:8622562-Receptors, Somatostatin,
pubmed-meshheading:8622562-Stomach Neoplasms,
pubmed-meshheading:8622562-Transcription, Genetic,
pubmed-meshheading:8622562-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Messenger RNA expression of somatostatin receptor subtypes in human and rat gastric mucosae.
|
| pubmed:affiliation |
Unité de Recherches Biologie et Pathogie de l'Epithelium Digestif, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|