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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1996-6-18
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pubmed:abstractText |
We developed new biochemical approaches to demonstrate the presence of inositol 1,4,5-triphosphate (InsP3)-gated calcium channels in presynaptic plasma membranes (SPM) and their involvement in the presynaptic receptor-mediated Ca2+ influx into nerve terminals. In perfusion experiments using SPM vesicles preloaded with 45Ca2+, InsP3 elicited the release of 45CA2+ into perfusates in a saturable manner. The InsP3- evoked 45Ca2+ release from resealed SPM vesicles was more potent than that from resealed vesicles using any other subcellular fractions. Here we also report the involvement of InsP3-gated mechanisms in the presynaptic receptor-mediated Ca2+ influx into synaptosomes (nerve terminals) by use of such resealed vesicles reconstituted with purified Gi1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Endorphins,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/kyotorphin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2891-900
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8622120-Adenosine Triphosphate,
pubmed-meshheading:8622120-Animals,
pubmed-meshheading:8622120-Biological Transport,
pubmed-meshheading:8622120-Calcimycin,
pubmed-meshheading:8622120-Calcium,
pubmed-meshheading:8622120-Calcium Channels,
pubmed-meshheading:8622120-Cell Membrane,
pubmed-meshheading:8622120-Endorphins,
pubmed-meshheading:8622120-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:8622120-Ion Channel Gating,
pubmed-meshheading:8622120-Kinetics,
pubmed-meshheading:8622120-Male,
pubmed-meshheading:8622120-Nerve Endings,
pubmed-meshheading:8622120-Presynaptic Terminals,
pubmed-meshheading:8622120-Rats,
pubmed-meshheading:8622120-Rats, Sprague-Dawley,
pubmed-meshheading:8622120-Subcellular Fractions,
pubmed-meshheading:8622120-Synaptic Vesicles,
pubmed-meshheading:8622120-Virulence Factors, Bordetella
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pubmed:year |
1996
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pubmed:articleTitle |
Inositol 1,4,5-trisphosphate-gated calcium transport through plasma membranes in nerve terminals.
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pubmed:affiliation |
Department of Pharmacology, Yokohama City University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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