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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-6-13
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pubmed:abstractText |
Surfactant protein A (SP-A) is an abundant lipoprotein component of pulmonary surfactant that plays multiple roles in surfactant homeostasis within the lung. A simple and rapid purification procedure for SP-A is described. Purified surfactant is washed by centrifugation with Ca2+ containing buffer to remove residual soluble proteins. Following the Ca2+ buffer wash, the surfactant pellet is washed in buffer containing EGTA and Mg2+ which releases the bound SP-A in almost pure form. Subsequent chromatography of the SP-A on Sephacryl S-500 yields homogeneous preparations of the protein. The SP-A purified using this procedure requires no exposure to either detergents or organic solvents to remove lipid. SP-A prepared by this new method inhibits lipid secretion from alveolar type II cells as effectively as SP-A prepared by other methods. In addition, the SP-A depleted surfactant produced in the first step of this procedure is capable of binding exogenous SP-A in a time dependent, saturable and Ca2+ dependent manner.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
327
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
285-91
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8619616-Animals,
pubmed-meshheading:8619616-Calcium,
pubmed-meshheading:8619616-Calcium Chloride,
pubmed-meshheading:8619616-Chromatography, Gel,
pubmed-meshheading:8619616-Egtazic Acid,
pubmed-meshheading:8619616-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8619616-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8619616-Kinetics,
pubmed-meshheading:8619616-Lung,
pubmed-meshheading:8619616-Molecular Weight,
pubmed-meshheading:8619616-Protein Binding,
pubmed-meshheading:8619616-Proteolipids,
pubmed-meshheading:8619616-Pulmonary Alveoli,
pubmed-meshheading:8619616-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:8619616-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:8619616-Pulmonary Surfactants,
pubmed-meshheading:8619616-Rats,
pubmed-meshheading:8619616-Rats, Sprague-Dawley
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pubmed:year |
1996
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pubmed:articleTitle |
Calcium dependent association of surfactant protein A with pulmonary surfactant: application to simple surfactant protein A purification.
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pubmed:affiliation |
Lord and Taylor Laboratory for Lung Biochemistry, Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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