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pubmed-article:8618913pubmed:abstractTextThe immunoglobulin kappa gene locus encodes 95% of the light chains of murine antibody molecules and is thought to contain up to 300 variable (V kappa)-region genes generally considered to comprise 20 families. To delineate the locus we have isolated 29 yeast artificial chromosome genomic clones that form two contigs, span > 3.5 megabases, and contain two known non-immunoglobulin kappa markers. Using PCR primers specific for 19 V kappa gene families and Southern analysis, we have refined the genetically defined order of these V kappa gene families. Of these, V kappa 2 maps at least 3.0 Mb from the joining (J kappa) region and appears to be the most distal V kappa gene segment.lld:pubmed
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pubmed-article:8618913pubmed:authorpubmed-author:LUGGlld:pubmed
pubmed-article:8618913pubmed:authorpubmed-author:GarrardW TWTlld:pubmed
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pubmed-article:8618913pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:8618913pubmed:articleTitleYeast artificial chromosome contigs reveal that distal variable-region genes reside at least 3 megabases from the joining regions in the murine immunoglobulin kappa locus.lld:pubmed
pubmed-article:8618913pubmed:affiliationMolecular Immunology Center, University of Texas Southwestern Medical Center, Dallas 75235-9140, USA.lld:pubmed
pubmed-article:8618913pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8618913pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:8618913pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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