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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
1996-6-7
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pubmed:abstractText |
The immunoglobulin kappa gene locus encodes 95% of the light chains of murine antibody molecules and is thought to contain up to 300 variable (V kappa)-region genes generally considered to comprise 20 families. To delineate the locus we have isolated 29 yeast artificial chromosome genomic clones that form two contigs, span > 3.5 megabases, and contain two known non-immunoglobulin kappa markers. Using PCR primers specific for 19 V kappa gene families and Southern analysis, we have refined the genetically defined order of these V kappa gene families. Of these, V kappa 2 maps at least 3.0 Mb from the joining (J kappa) region and appears to be the most distal V kappa gene segment.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1427923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1475190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1525825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1557040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1596359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1645798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1779860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1794048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1910683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-1976702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2110660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2115125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2123475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2563357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2574159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2898504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-2998983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-3002631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-3116501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-3138071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-3937727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-6411613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-6420501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-7540588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-7546385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-7749979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-7920646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-8075633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-8276255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8618913-8358173
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12421-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8618913-Animals,
pubmed-meshheading:8618913-Base Sequence,
pubmed-meshheading:8618913-Blotting, Southern,
pubmed-meshheading:8618913-Chimera,
pubmed-meshheading:8618913-Chromosome Mapping,
pubmed-meshheading:8618913-Chromosomes, Artificial, Yeast,
pubmed-meshheading:8618913-DNA Primers,
pubmed-meshheading:8618913-Gene Library,
pubmed-meshheading:8618913-Genes, Immunoglobulin,
pubmed-meshheading:8618913-Immunoglobulin Joining Region,
pubmed-meshheading:8618913-Immunoglobulin Variable Region,
pubmed-meshheading:8618913-Immunoglobulin kappa-Chains,
pubmed-meshheading:8618913-Introns,
pubmed-meshheading:8618913-Mice,
pubmed-meshheading:8618913-Molecular Sequence Data,
pubmed-meshheading:8618913-Multigene Family,
pubmed-meshheading:8618913-Polymerase Chain Reaction,
pubmed-meshheading:8618913-Restriction Mapping
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pubmed:year |
1995
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pubmed:articleTitle |
Yeast artificial chromosome contigs reveal that distal variable-region genes reside at least 3 megabases from the joining regions in the murine immunoglobulin kappa locus.
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pubmed:affiliation |
Molecular Immunology Center, University of Texas Southwestern Medical Center, Dallas 75235-9140, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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