Linked Life Data

8617713

Source:http://linkedlifedata.com/resource/pubmed/id/8617713

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1996-6-11
pubmed:abstractText
The mercurial insensitive water channel (MIWC, AQP-4) is a water-selective transporter expressed at the basolateral plasma membrane of principal cells in kidney collecting duct, airway epithelium, and gastric parietal cells, as well as in astrocytes and skeletal muscle plasmalemma. Because these sites correspond to membranes where orthogonal arrays of particles (OAPs) have been observed by freeze-fracture electron microscopy, we tested the hypothesis that MIWC forms OAPs. Chinese hamster ovary cells were stably transfected with the coding sequence of rat MIWC under a cytomegalovirus promoter. Immunostaining of clonal cell populations showed MIWC expression at the plasma membrane. A single band at 31 kDa was detected on immunoblot. Cell fractionation by sucrose gradient centrifugation indicated strong MIWC expression in plasma membrane fractions with lesser expression in Golgi. Functional analysis by stopped-flow light scattering showed high mercurial insensitive water permeability in plasma membrane vesicles. Freeze-fracture electron microscopy showed distinct OAPs on the plasma membrane P-face of MIWC-expressing cells with morphology indistinguishable from that in basolateral membrane of kidney collecting duct; the E-face showed corresponding linear grooves (spacing, approximately 8 nm) in transfected cells and collecting duct. OAPs were not observed in control (empty vector-transfected) cells or CHIP28 (AQP1)-transfected cells in which disorganized intramembrane particle aggregates were found. These results provide direct evidence that a molecular water channel can spontaneously assemble in regular arrays.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4577-80
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8617713-Animals, pubmed-meshheading:8617713-Aquaporin 1, pubmed-meshheading:8617713-Aquaporin 4, pubmed-meshheading:8617713-Aquaporins, pubmed-meshheading:8617713-Base Sequence, pubmed-meshheading:8617713-CHO Cells, pubmed-meshheading:8617713-Cell Membrane, pubmed-meshheading:8617713-Cricetinae, pubmed-meshheading:8617713-DNA Primers, pubmed-meshheading:8617713-Dogs, pubmed-meshheading:8617713-Freeze Fracturing, pubmed-meshheading:8617713-Ion Channels, pubmed-meshheading:8617713-Kidney, pubmed-meshheading:8617713-Lung, pubmed-meshheading:8617713-Microscopy, Electron, pubmed-meshheading:8617713-Molecular Sequence Data, pubmed-meshheading:8617713-Polymerase Chain Reaction, pubmed-meshheading:8617713-Rats, pubmed-meshheading:8617713-Recombinant Proteins, pubmed-meshheading:8617713-Templates, Genetic, pubmed-meshheading:8617713-Transfection, pubmed-meshheading:8617713-Water-Electrolyte Balance
pubmed:year
1996
pubmed:articleTitle
The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells.
pubmed:affiliation
Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't