Linked Life Data

8617376

Source:http://linkedlifedata.com/resource/pubmed/id/8617376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-6-7
pubmed:abstractText
We examined the isoenzyme patterns of alpha and beta naphtyl acetate esterase and the IL6 production of two macrophage cell lines, which were cloned from a single fusion of macrophage tumor cells and spleen adherent cells. These clones were phenotypically indistinguishable but differ functionally as line 59 presents antigen to Th 1 lymphocytes while line 63 induces suppressor T lymphocytes. Cell extracts of these lines exhibit different isoenzyme patterns of alpha and beta naphtyl acetate esterase at both pH 7.5 and 5.8 but do not differ significantly in the level of produced IL6. Treatment with nitrogranulogen (NG), a derivative of cyclophosphamide, changes the isoenzyme pattern in line 59 and decreases severalfold IL6 production, while in similarly treated line 63 cells isoenzyme pattern remains unaffected but the production of IL6 is significantly increases. We assume that the observed differences between these two cell lines are due to distinct intracellular translation of the membrane signal delivered by NG. The different behaviour of these two parameters can thus be used as a useful tool to further delineate different macrophage subpopulations. We regard it likely that nonspecific esterases may play a role in intracellular processing or trafficking of antigen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0239-8508
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Different isoenzyme patterns of nonspecific esterases and the level of IL6 production as markers of macrophage functions.
pubmed:affiliation
Department of Immunology, Jagiellonian University, Collegium Medicum, Cracow, Poland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't