Linked Life Data

8615609

Source:http://linkedlifedata.com/resource/pubmed/id/8615609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-6-5
pubmed:abstractText
Ki67 expression, S-phase fraction, p53 immunoreactivity and DNA content were examined in morphologically normal mucosa and squamous dysplasia of both cancerous and non-cancerous human oesophagi in order to understand possible early events in the development of esophageal squamous cell carcinoma. 103 different foci from cancerous esophagi including 17 non-pathological epithelium, 10 mild, 17 moderate and 15 severe dysplasia, 14 intraepithelial carcinomas and 30 invasive squamous cell carcinomas were examined. Also studied were 57 biopsy specimens from cancer-free individuals, including 12 normal epithelia, 15 oesophagitis, and 16 mild, 11 moderate and 3 severe dysplasia. Areas of squamous dysplasia from both cancer-free and cancerous oesophagi were morphologically indistinguishable and both demonstrated increased cellular proliferation compared to normal or non-pathological epithelia. However, squamous dysplasia in cancerous oesophagi demonstrated significantly larger ki67 labelling indices and smaller S-phase fractions than dysplasia in cancer-free patients. Squamous dysplasia in cancerous and non-cancerous oesophagi demonstrated an non-diploid DNA histogram in 67.9% and 43.3% respectively. However, dysplasia from cancer-free individuals demonstrated a non-diploid pattern with one or more peaks (Type I non-diploid histogram) and that from oesophageal cancer patients predominantly exhibited non-diploid histograms without any distinctive peaks (Type II non-diploid histogram). Significant differences in the frequency of p53 positive foci were observed between dysplasia of cancer-free (23.3%) and cancerous (56.8%) oesophagi. IN cancerous oesophagi, dysplasia associated with Type II non-diploid histograms had a significantly larger number of p53-positive foci than those with diploid histograms or Type I non-diploid histograms. These results indicated that the biological features of squamous dysplasia were different between cancerous and non-cancerous human oesophagi despite indistinguishable morphological features. In addition, the combination of p53 immuno-histochemistry and DNA ploidy analysis may contribute to identify possible high-risk squamous dysplasia of the oesophagus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8615609-Adult, pubmed-meshheading:8615609-Aged, pubmed-meshheading:8615609-Aged, 80 and over, pubmed-meshheading:8615609-Carcinoma, Squamous Cell, pubmed-meshheading:8615609-Cell Division, pubmed-meshheading:8615609-DNA, Neoplasm, pubmed-meshheading:8615609-Epithelial Cells, pubmed-meshheading:8615609-Epithelium, pubmed-meshheading:8615609-Esophageal Neoplasms, pubmed-meshheading:8615609-Esophagus, pubmed-meshheading:8615609-Female, pubmed-meshheading:8615609-Humans, pubmed-meshheading:8615609-Immunohistochemistry, pubmed-meshheading:8615609-Ki-67 Antigen, pubmed-meshheading:8615609-Male, pubmed-meshheading:8615609-Middle Aged, pubmed-meshheading:8615609-Neoplasm Proteins, pubmed-meshheading:8615609-Nuclear Proteins, pubmed-meshheading:8615609-Ploidies, pubmed-meshheading:8615609-Precancerous Conditions, pubmed-meshheading:8615609-S Phase, pubmed-meshheading:8615609-Tumor Suppressor Protein p53
pubmed:articleTitle
DNA ploidy, P53 expression, and cellular proliferation in normal epithelium and squamous dysplasia of non-cancerous and cancerous human oesophagi.
pubmed:affiliation
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
pubmed:publicationType
Journal Article, Comparative Study