Linked Life Data

8612546

Source:http://linkedlifedata.com/resource/pubmed/id/8612546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1996-6-5
pubmed:abstractText
It has been known for many years that adipocytes express high affinity ACTH and alpha-melanocyte stimulating hormone (MSH) binding sites, and that ACTH, alpha-MSH, and beta-lipotropin are potent lipolytic hormones. We show here that the adipocyte response to the melanocortin peptides results from the expression of both the MC2 (ACTH) receptor as well as the newly discovered MC5 receptor. Using RT-PCR and Northern blot hybridization, high levels of MC2 receptor messenger RNA (mRNA) were found in all adipose tissues examined in the mouse, whereas MC5 receptor mRNA was found in a subset of these. Both receptors mRNAs were also found in the 3T3-L1 cell line but only after the cells had been induced to differentiate into adipocytes. This cell line was then used to characterize the pharmacological properties of the MC2 and MC5 receptor sites in situ. The MC2 receptor exhibits properties similar to the ACTH receptor characterized in adrenocortical cells, coupling to activation of adenylyl cyclase with an EC50 of approximately 1 nM. An MSH binding site characterized in these cells is presumably the MC5 receptor, based on the observation that this is the only other melanocortin receptor mRNA detected in these cells. The MC5 receptor in the 3T3-L1 adipocyte activated adenylyl cyclase in response to alpha-MSH stimulation. Interestingly, Nle4, D-Phe7-alpha-MSH (NDP-MSH), a commonly used synthetic alpha-MSH agonist, was a potent antagonist of the MC5 receptor expressed in the 3T3-L1 cell line. Although the agouti signaling peptide is a potent antagonist of NDP-MSH binding to the MC1 and MC4 melanocortin receptors, agouti was unable to block NDP-MSH binding in the 3T3-L1 adipocyte.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2043-50
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8612546-3T3 Cells, pubmed-meshheading:8612546-Adenylate Cyclase, pubmed-meshheading:8612546-Adipocytes, pubmed-meshheading:8612546-Adipose Tissue, pubmed-meshheading:8612546-Agouti Signaling Protein, pubmed-meshheading:8612546-Animals, pubmed-meshheading:8612546-Base Sequence, pubmed-meshheading:8612546-Blotting, Northern, pubmed-meshheading:8612546-Gene Expression, pubmed-meshheading:8612546-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:8612546-Melanocyte-Stimulating Hormones, pubmed-meshheading:8612546-Mice, pubmed-meshheading:8612546-Molecular Sequence Data, pubmed-meshheading:8612546-Polymerase Chain Reaction, pubmed-meshheading:8612546-Proteins, pubmed-meshheading:8612546-RNA, Messenger, pubmed-meshheading:8612546-Receptors, Corticotropin, pubmed-meshheading:8612546-Receptors, Melanocortin, pubmed-meshheading:8612546-alpha-MSH
pubmed:year
1996
pubmed:articleTitle
Characterization of melanocortin receptor subtype expression in murine adipose tissues and in the 3T3-L1 cell line.
pubmed:affiliation
Department of Pediatrics, Oregon Health Sciences University, Portland 97201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.