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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1996-5-29
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pubmed:abstractText |
Human Langerhans cells (LC) are CD1a+ dendritic cells (DC) that function as potent antigen-presenting cells for primary and secondary immune responses. Limitations in DC/LC numbers, imposed by difficult and tedious isolation procedures, have so far precluded their use as immunogens in the generation and/or augmentation of host responses against various pathogens. Therefore, we have developed a procedure for the generation of human DC/LC from CD34+ hematopoietic progenitor cells (HPC) isolated (mean: 0.7 x 10(6)/ buffy coat and 2.6 x 10(6)/leukapheresis product) and purified ( > 95%) from the peripheral blood of healthy adults. In vitro stimulation of these cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha led to their vigorous proliferation and differentiation resulting in the emergence of CD45+/CD68+/CD3-/CD19-/CD56- leukocytes some of which (mean: 12%) express CD1a and exhibit anti-CD4 and anti-major histocompatibility complex (MHC) class II reactivity. These CD1a- leukocytes include (1) LC as evidenced by the presence of Birbeck granules (BG), (2) CD14+ monocytes, and (3) Birbeck granule-negative cells with a dendritic morphology. Addition of interleukin (IL)-4 to the cytokine cocktail interfered with the development of monocytes and led to a reduction in the overall yield but, on the other hand, resulted in an increased percentage of CD1a+ cells (mean: 24%) among all cells generated. In vitro generated CD1a+, but not CD1a- HPC-derived cells are potent stimulators of the primary mixed leukocyte reaction and, as such, promising candidates for vaccination purposes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1292-302
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8608217-Adult,
pubmed-meshheading:8608217-Antigens, CD1,
pubmed-meshheading:8608217-Antigens, CD34,
pubmed-meshheading:8608217-Blood Cells,
pubmed-meshheading:8608217-Cell Division,
pubmed-meshheading:8608217-Cell Separation,
pubmed-meshheading:8608217-Cells, Cultured,
pubmed-meshheading:8608217-Dendritic Cells,
pubmed-meshheading:8608217-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:8608217-Hematopoietic Stem Cells,
pubmed-meshheading:8608217-Humans,
pubmed-meshheading:8608217-Interleukin-4,
pubmed-meshheading:8608217-Langerhans Cells,
pubmed-meshheading:8608217-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:8608217-Lymphocyte Depletion,
pubmed-meshheading:8608217-Tumor Necrosis Factor-alpha
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pubmed:year |
1996
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pubmed:articleTitle |
Generation of human dendritic cells/Langerhans cells from circulating CD34+ hematopoietic progenitor cells.
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pubmed:affiliation |
Division of Immunology, Allergy and Infectious Diseases, Vienna International Research Cooperation Center (VIRCC), University of Vienna Medical School, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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