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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-5-21
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pubmed:abstractText |
The expression of two small basic proteins (C and C') encoded by a second open reading frame of the New Jersey serotype of vesicular stomatitis virus (VSV) P gene was reported previously (Spiropoulou and Nichol, J. Virol., 67, 3103-3110, 1993). Here we found that the Indiana serotype virus also expressed C and C' proteins from this reading frame. We eliminated C and C' expression by making a single base change that introduced a stop codon in the C and C' coding sequence, but left the P-protein sequence unchanged. This mutated P gene supported normal replication and packaging of VSV minigenomes encoding G and M proteins. The mutated P gene was also recombined into an infectious clone of VSV that was used to recover virus. The mutant virus no longer expressed the C and C' proteins but showed growth kinetics identical to wild-type virus. The amounts of viral mRNAs and proteins synthesized were indistinguishable in mutant and wild-type virus infected cells as were the yields and composition of mutant and wild-type virus particles. The kinetics of host protein-synthesis shut-off were also identical for both viruses. Although the C and C' proteins were dispensable for VSV growth in tissue culture, they are known to be conserved in all vesiculoviruses, and thus perhaps play a role in viral pathogenesis or transmission by insect vectors.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0042-6822
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
216
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
309-16
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8607260-Amino Acid Sequence,
pubmed-meshheading:8607260-Animals,
pubmed-meshheading:8607260-Base Sequence,
pubmed-meshheading:8607260-Blotting, Northern,
pubmed-meshheading:8607260-Cricetinae,
pubmed-meshheading:8607260-DNA, Viral,
pubmed-meshheading:8607260-Genome, Viral,
pubmed-meshheading:8607260-Molecular Sequence Data,
pubmed-meshheading:8607260-Point Mutation,
pubmed-meshheading:8607260-Rabbits,
pubmed-meshheading:8607260-Time Factors,
pubmed-meshheading:8607260-Transcription, Genetic,
pubmed-meshheading:8607260-Vesicular stomatitis Indiana virus,
pubmed-meshheading:8607260-Vesiculovirus,
pubmed-meshheading:8607260-Viral Nonstructural Proteins,
pubmed-meshheading:8607260-Viral Structural Proteins,
pubmed-meshheading:8607260-Virus Replication
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pubmed:year |
1996
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pubmed:articleTitle |
Normal replication of vesicular stomatitis virus without C proteins.
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pubmed:affiliation |
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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