Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1996-5-20
|
pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S81566,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S81570,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S81573,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S81574,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S81575
|
pubmed:abstractText |
A large panel of T cell clones (TCC) specific for the recombinant form of Poa pratensis allergen (rKBG7.2 or Poa p9) were established from the peripheral blood of grass pollen-sensitive donor in the absence or presence of recombinant interferon-alpha (IFN-alpha) in bulk culture and their pattern of cytokine secretion, peptide reactivity and TCR V beta repertoire was examined. The majority of allergen-specific TCC derived in absence of IFN-alpha produced high amounts of interleukin-4 (IL-4) and IL-5 but not IFN-gamma (Th2 cells), while most of TCC derived in presence of IFN-alpha produced IFN-gamma but not, or limited amounts of, IL-4 and IL-5 (Th1 or Th0 cells). Of 24 TCC established in the presence of IFN-alpha, 22 were able to recognize a single allergen peptide, p26, while none of the clones established in the absence of IFN-alpha showed a similar specificity. The majority of both clones expressed the V beta 2 element regardless of whether they were established in the presence of INF-alpha, but the presence of IFN-alpha favored the expansion of V beta 2+, V beta 17+ and V beta 22+ Poa p9-specific T cells, whereas in the absence of IFN-alpha, other TCR V beta-bearing T cells (V beta 5, and V beta 6.7, and V beta 14) were expanded in addition to V beta 2+ T cells. None of V beta 2+ clones established in the absence of IFN-alpha reacted with p26, whereas all the V beta 2+ clones established in its presence in the absence of interferon-alpha reacted with p26, whereas all the V beta 2+ clones established in its presence reacted to this peptide. IFN-alpha also shifted the TCR V beta repertoire of both Poa p9- and Lolium perenne group 1 (Lol p1)-specific T cell lines generated from the same patient and from a different grass-sensitive individual. These data demonstrate that IFN-alpha modulates the development of allergen-specific T cells in vitro, and suggest that IFN-alpha may represent a useful tool for novel immunotherapeutic approaches in allergic disorders.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Allergens,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lol p I protein, Lolium perenne,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Poa p I proteins, Poa pratensis,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0014-2980
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
26
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
697-703
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:8605940-Allergens,
pubmed-meshheading:8605940-Base Sequence,
pubmed-meshheading:8605940-Clone Cells,
pubmed-meshheading:8605940-Cytokines,
pubmed-meshheading:8605940-Epitopes,
pubmed-meshheading:8605940-Humans,
pubmed-meshheading:8605940-Interferon-alpha,
pubmed-meshheading:8605940-Interferon-gamma,
pubmed-meshheading:8605940-Interleukin-4,
pubmed-meshheading:8605940-Molecular Sequence Data,
pubmed-meshheading:8605940-Peptides,
pubmed-meshheading:8605940-Plant Proteins,
pubmed-meshheading:8605940-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8605940-T-Lymphocytes
|
pubmed:year |
1996
|
pubmed:articleTitle |
Effects of interferon-alpha on cytokine profile, T cell receptor repertoire and peptide reactivity of human allergen-specific T cells.
|
pubmed:affiliation |
Division of Clinical Immunology and Allergy, University of Florence, Italy.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|