Linked Life Data

8605345

Source:http://linkedlifedata.com/resource/pubmed/id/8605345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1996-5-17
pubmed:abstractText
Surface IgM+B220+ B cell precursors can be categorized as either leukosialin (CD43/S7) negative (late stage pre-B cells) or positive (pro-B/early pre-B cells). In autoimmune New Zealand Black (NZB) mice, bone marrow small pre-B cells (IgM-CD43-B220+) and pro-B/early pre-B cells (IgM-CD43+B220+) declined significantly with age. In particular, subpopulations of pro-B/early pre-B cells expressing the heat stable antigen (HSA) were found in lower proportions with age. Significant decreases in interleukin-7 (IL-7) colony forming units (CFU) were also seen in NZB mice by 6 to 8 months of age and accompanied alterations in the numbers of pro-B and pre-B cells in bone marrow. Concomitant with reduced numbers of B lineage precursor cells and IL-7 CFU in vivo, NZB mice produced serum IgM antibodies that strongly inhibited IL-7 CFU responses in vitro. Two monoclonal IgM antibodies (5G9, 2F5) derived from LPS stimulated 10-month-old NZB splenocytes recognized pre-B cell surface antigens on both pre-B cell lines and on IL-7 stimulated bone marrow pro-B/pre-B cells. However, these monoclonal antibodies (MoAb) failed to significantly stain ex vivo bone marrow cells. The 5G9 and 2F5 MoAbs also partially inhibited IL-7 CFU in vitro. These results suggest that NZB bone marrow becomes increasingly deficient in B cell precursors and especially in IL-7 responsive pre-B cells with age. IgM serum antibodies and monoclonal IgM antibodies derived from older NZB mice inhibit pre-B cell growth to IL-7. The production of such autoantibodies may interfere with B cell development in aging NZB mice by preventing IL-7-mediated proliferation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3289-96
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8605345-Age Factors, pubmed-meshheading:8605345-Animals, pubmed-meshheading:8605345-Antibodies, Monoclonal, pubmed-meshheading:8605345-Antigens, CD, pubmed-meshheading:8605345-Antigens, CD43, pubmed-meshheading:8605345-Autoantibodies, pubmed-meshheading:8605345-Autoimmune Diseases, pubmed-meshheading:8605345-B-Lymphocytes, pubmed-meshheading:8605345-Bone Marrow, pubmed-meshheading:8605345-Cell Death, pubmed-meshheading:8605345-Cell Differentiation, pubmed-meshheading:8605345-Cell Division, pubmed-meshheading:8605345-Cells, Cultured, pubmed-meshheading:8605345-Colony-Forming Units Assay, pubmed-meshheading:8605345-Hematopoietic Stem Cells, pubmed-meshheading:8605345-Immunoglobulin M, pubmed-meshheading:8605345-Interleukin-7, pubmed-meshheading:8605345-Mice, pubmed-meshheading:8605345-Mice, Inbred BALB C, pubmed-meshheading:8605345-Mice, Inbred NZB, pubmed-meshheading:8605345-Sialoglycoproteins
pubmed:year
1996
pubmed:articleTitle
Autoantibodies inhibit interleukin-7-mediated proliferation and are associated with the age-dependent loss of pre-B cells in autoimmune New Zealand Black Mice.
pubmed:affiliation
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33101, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.