Linked Life Data

8604293

Source:http://linkedlifedata.com/resource/pubmed/id/8604293

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-5-15
pubmed:abstractText
Transcription from a minimal HIV-1 promoter containing the three Sp1 binding sites and TATA box can be activated without Tat by template DNA replication. Here we show that this activation can also be mediated by recombinant GAL4 fusion proteins containing the activation domains of Sp1, VP16 or CTF (or by full-length GAL4) targeted to the HIV-1 promoter by replacing the Sp1 sites with five GAL4 binding sites. Thus Sp1 is not unique in its ability to mediate replication activated transcription, although the degree of processivity elicited by the different activators varied significantly from strongly processive (GAL4-VP16) to relatively non-processive (GAL4-Sp1 or -CTF). Processive GAL4-VP16-activated transcription, but not efficient initiation, required multiple GAL4 binding sites. In the presence of Tat, transcription with GAL4-SP1 and GAL4-CTF was further activated (principally at the level of processivity) but GAL4-VP16-potentiated transcription was only slightly stimulated. The Tat-dependent switch from non-processive to fully processive transcription was particularly marked for GAL4-Sp1, an effect which may be relevant to the selection of Sp1 binding sites by the HIV-1 promoter.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1322294, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1464321, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1464322, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1535687, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1583736, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1622932, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1658792, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1727476, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1752440, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-1885667, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2038313, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2153460, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2160609, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2538719, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2549858, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2798115, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2828163, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2830022, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-2835669, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-3025639, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-3028647, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-3031591, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-3044607, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-3142690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-3288969, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-6086311, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-6091052, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-6200874, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-6276753, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7448872, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7529174, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7690421, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7698646, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7815509, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7856090, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-7979253, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8035798, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8205623, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8227056, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8246964, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8370526, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8414985, http://linkedlifedata.com/resource/pubmed/commentcorrection/8604293-8431942
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
549-57
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Activation domains of transcription factors mediate replication dependent transcription from a minimal HIV-1 promoter.
pubmed:affiliation
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't