Linked Life Data

8603104

Source:http://linkedlifedata.com/resource/pubmed/id/8603104

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-5-15
pubmed:databankReference
pubmed:abstractText
Two phosphatidylinositol 4-kinase isozymes, type 3 and type 2, have been separated on hydroxylapatite after solubilizing bovine brain microsomes with Triton X-114. Employing a newly developed renaturation procedure following SDS-PAGE, we demonstrate that a 200 kDa polypeptide carries the enzyme activity of this type 3 isoform. Chromatography on hydroxylapatite, Heparin-Sepharose, Superdex 200 and finally SDS-PAGE results in an approximately 30,000-fold purification. Tryptic peptides generated from the 200 kDa polypeptide after SDS-PAGE have been sequenced and the obtained data have been used for constructing and synthesizing degenerated oligonucleotides. Polymerase chain reaction as well as screening of cDNA libraries allowed several clones to be isolated from which a 4.7 kb contiguous sequence can be built up. The open reading frame covers 4.4 kb with a 0.3 kb untranslated 3' end which yields a deduced amino acid sequence of 1,467 amino acids. The C-terminal part of ca. 300 amino acids represents the catalytic domain. Sequence alignment of this domain with the mammalian counterpart, the human type 2 phosphatidylinositol 4-kinase, the yeast kinases STT4 and PIK1, as well as with the catalytic domains of bovine, human, mouse and yeast phosphatidylinositol 3-kinases reveals a high degree of identity: 26 of these approximately 300 amino acids are invariable in all of these eight catalytic domains. Five motifs indicate nuclear localization and DNA binding properties of the enzyme. Two leucine zipper motifs (amino acids 358-386, 862-882) are detectable. Furthermore, a helix loop helix motif (amino acids 716-729) as well as two nuclear localization signals (amino acids 838-854, 345-349) indicate the presence of the type 3 isoform in the nucleus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
1311
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-63
pubmed:dateRevised
2007-12-11
pubmed:meshHeading
pubmed-meshheading:8603104-1-Phosphatidylinositol 4-Kinase, pubmed-meshheading:8603104-Amino Acid Sequence, pubmed-meshheading:8603104-Animals, pubmed-meshheading:8603104-Base Sequence, pubmed-meshheading:8603104-Brain, pubmed-meshheading:8603104-Cattle, pubmed-meshheading:8603104-Cell Nucleus, pubmed-meshheading:8603104-DNA, Complementary, pubmed-meshheading:8603104-DNA Primers, pubmed-meshheading:8603104-Humans, pubmed-meshheading:8603104-Mice, pubmed-meshheading:8603104-Microsomes, pubmed-meshheading:8603104-Molecular Sequence Data, pubmed-meshheading:8603104-Molecular Weight, pubmed-meshheading:8603104-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:8603104-Saccharomyces cerevisiae Proteins, pubmed-meshheading:8603104-Sequence Alignment, pubmed-meshheading:8603104-Sequence Analysis, pubmed-meshheading:8603104-Sequence Homology, Amino Acid
pubmed:year
1996
pubmed:articleTitle
Identification of a 200 kDa polypeptide as type 3 phosphatidylinositol 4-kinase from bovine brain by partial protein and cDNA sequencing.
pubmed:affiliation
Ruhr-Universität Bochum, Institut für Physiologische Chemie, Abteilung für Biochemie Supramolekularer Systeme, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't