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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1996-5-6
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pubmed:abstractText |
The presence of BCL-2 gene rearrangement has been detected also in cellular populations lacking the t(14;18) chromosomal translocation, such as B-lineage acute lymphoblastic leukemia (ALL) cells. It has been reported that overexpression of BCL-2 is related to resistance to glucocorticoid-induced apoptosis. In this study, we aimed to evaluate whether in vitro culture with prednisolone (PDN) could modify the expression of BCL-2 protein. ALL cells from 21 patients were incubated for 72 hr with or without a minimally lethal (IC12) dose of PDN. In vitro culture with PDN did not affect the percentage of positive cells, even though the mean fluorescence index was significantly increased (P = 0.0001), thus indicating a higher level of protein production. These data could suggest a possible mechanism of drug resistance after treatment with PDN.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0361-8609
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
261-4
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8602624-Adolescent,
pubmed-meshheading:8602624-Adult,
pubmed-meshheading:8602624-Aged,
pubmed-meshheading:8602624-Antineoplastic Combined Chemotherapy Protocols,
pubmed-meshheading:8602624-Apoptosis,
pubmed-meshheading:8602624-Asparaginase,
pubmed-meshheading:8602624-Bone Marrow,
pubmed-meshheading:8602624-Burkitt Lymphoma,
pubmed-meshheading:8602624-Cyclophosphamide,
pubmed-meshheading:8602624-Doxorubicin,
pubmed-meshheading:8602624-Drug Resistance, Neoplasm,
pubmed-meshheading:8602624-Female,
pubmed-meshheading:8602624-Gene Expression Regulation, Leukemic,
pubmed-meshheading:8602624-Humans,
pubmed-meshheading:8602624-Male,
pubmed-meshheading:8602624-Middle Aged,
pubmed-meshheading:8602624-Neoplasm Proteins,
pubmed-meshheading:8602624-Prednisolone,
pubmed-meshheading:8602624-Proto-Oncogene Proteins,
pubmed-meshheading:8602624-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8602624-Tumor Cells, Cultured,
pubmed-meshheading:8602624-Vincristine
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pubmed:year |
1996
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pubmed:articleTitle |
In vitro culture with prednisolone increases BCL-2 protein expression in adult acute lymphoblastic leukemia cells.
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pubmed:affiliation |
Institute of Hematology, University of Bologna, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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