Linked Life Data

8600936

Source:http://linkedlifedata.com/resource/pubmed/id/8600936

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-5-8
pubmed:abstractText
Acute inhalation of the pulmonary irritant ozone is associated with an inflammatory response characterized by increased numbers of macrophages in the lung that release elevated quantities of nitric oxide. The accumulation of phagocytes in the lung is dependent on expression of leukocyte adhesion molecules including Mac-1. In the present studies, we determined whether activation of the Mac-1 receptor is involved in regulating nitric oxide production by lung phagocytes, and whether this response is modified following acute ozone inhalation. Cells were isolated from the lung by bronchoalveolar lavage 48 h after exposure of female Sprague-Dawley rats to air or ozone (2 parts per million, for 3 h). Anti-Mac-1beta antibody, but not anti-Mac-1alpha antibody, stimulated nitric oxide production by cells from both air- and ozone-exposed animals. Cells from ozone-exposed rats produced more nitric oxide and expressed greater quantities of inducible nitric oxide synthase mRNA than did cells from air-exposed animals. Production of nitric oxide in response to anti-Mac-1beta was also found to be augmented by cross-linking of the Mac-1beta receptor. Pretreatment of lavage cells with granulocyte/macrophage colony-stimulating factor (GM-CSF), which activates phagocytes, enhanced the expression of Mac-1beta and increased anti-Mac-1beta-induced nitric oxide production by the cells. Lavage cells from ozone-exposed animals were more responsive to GM-CSF than were cells from control animals. Taken together, these data suggest that the Mac-1beta adhesion molecule may contribute to phagocyte activation and mediator release during ozone-induced inflammatory reactions in the lung.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Stimulation of nitric oxide production in rat lung lavage cells by anti-Mac-1beta antibody: effects of ozone inhalation.
pubmed:affiliation
Department of Pharmacology and Toxicology, Rutgers University, Piscataway, New Jersey 08855-0789, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't