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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1996-5-1
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pubmed:abstractText |
RNase P, an enzyme essential for tRNA biosynthesis, can be directed to cleave any RNA when the target RNA is in a complex with a short, complementary oligonucleotide called an external guide sequence (EGS). RNase P from Escherichia coli can cleave phage lambda N mRNA in vitro or in vivo when the mRNA is in a complex with an EGS. The EGS can either be separate from or covalently linked to M1 RNA, the catalytic RNA subunit of RNase P. The requirement for Mg2+ in the reaction in vitro is lower when the EGS is covalently linked to M1 RNA. Substrates made of DNA can also be cleaved by RNase P in vitro in complexes with RNA EGSs. When either kind of EGS construct is used in vivo, burst size of phage lambda is reduced by > or = 40%. Reduction in burst size depends on efficient expression of the EGS constructs. The product of phage lambda gene N appears to function in a stoichiometric fashion.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-1280996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-1373488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-1381505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-1697102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-1741379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-1871136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-2432595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-2459398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-2471397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-2480641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-2482430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-3315856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-3537305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-3549453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-7479948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-7533740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-7539787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-7975207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-8075082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8600449-8233817
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Catalytic,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease P,
http://linkedlifedata.com/resource/pubmed/chemical/ribonuclease P, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
835-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8600449-Bacteriophage lambda,
pubmed-meshheading:8600449-Base Sequence,
pubmed-meshheading:8600449-Endoribonucleases,
pubmed-meshheading:8600449-Escherichia coli,
pubmed-meshheading:8600449-Escherichia coli Proteins,
pubmed-meshheading:8600449-Genes, Viral,
pubmed-meshheading:8600449-Molecular Sequence Data,
pubmed-meshheading:8600449-RNA, Catalytic,
pubmed-meshheading:8600449-RNA, Messenger,
pubmed-meshheading:8600449-RNA, Viral,
pubmed-meshheading:8600449-Ribonuclease P
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pubmed:year |
1996
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pubmed:articleTitle |
Cleavage by RNase P of gene N mRNA reduces bacteriophage lambda burst size.
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pubmed:affiliation |
Department of Biology, Yale University, New Haven, CT 06520, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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