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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5255
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pubmed:dateCreated |
1996-4-25
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pubmed:abstractText |
In the first step of V(D)J recombination, the RAG1 and RAG2 proteins cleave DNA between a signal sequence and the adjacent coding sequence, generating a blunt signal end and a coding end with a closed hairpin structure. These hairpins are intermediates leading to the formation of assembled antigen receptor genes. It is shown here that the hairpins are formed by a chemical mechanism of direct trans-esterification, very similar to the early steps of transpositional recombination and retroviral integration. A minor variation in the reaction is sufficient to divert the process from transposition to hairpin formation.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RAG-1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinases,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/V(D)J recombination activating...,
http://linkedlifedata.com/resource/pubmed/chemical/VDJ Recombinases,
http://linkedlifedata.com/resource/pubmed/chemical/integron integrase IntI1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1592-4
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:8599117-Base Sequence,
pubmed-meshheading:8599117-DNA,
pubmed-meshheading:8599117-DNA Nucleotidyltransferases,
pubmed-meshheading:8599117-DNA Transposable Elements,
pubmed-meshheading:8599117-DNA-Binding Proteins,
pubmed-meshheading:8599117-Esterification,
pubmed-meshheading:8599117-Gene Rearrangement,
pubmed-meshheading:8599117-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:8599117-Genes, Immunoglobulin,
pubmed-meshheading:8599117-HIV,
pubmed-meshheading:8599117-Homeodomain Proteins,
pubmed-meshheading:8599117-Integrases,
pubmed-meshheading:8599117-Molecular Sequence Data,
pubmed-meshheading:8599117-Nucleic Acid Conformation,
pubmed-meshheading:8599117-Proteins,
pubmed-meshheading:8599117-Recombinases,
pubmed-meshheading:8599117-Recombination, Genetic,
pubmed-meshheading:8599117-Thionucleotides,
pubmed-meshheading:8599117-VDJ Recombinases,
pubmed-meshheading:8599117-Virus Integration
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pubmed:year |
1996
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pubmed:articleTitle |
Similarities between initiation of V(D)J recombination and retroviral integration.
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pubmed:affiliation |
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0540, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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