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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6569
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pubmed:dateCreated |
1996-4-22
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pubmed:databankReference |
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pubmed:abstractText |
A large multisubunit complex containing RNA polymerase II, general transcription factors and SRB regulatory proteins initiates transcription of class II genes in yeast cells. The SRB proteins are a hallmark of this RNA polymerase II holoenzyme as they are found only in this complex, where they contribute to the response to regulators. We have now isolated a human homologue of the yeast SRB7 gene and used antibodies against human SRB7 protein to purify and characterize a mammalian RNA polymerase II holoenzyme containing the general transcription factors TFIIE and TFIIH. This holoenzyme is more responsive to transcriptional activators than core RNA polymerase II when assayed in the presence of coactivators.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mediator Complex,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/SRB7 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAF6 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Binding Protein Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIID,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIIH,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, TFII,
http://linkedlifedata.com/resource/pubmed/chemical/transcription factor TFIIE
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
380
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
82-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8598913-Amino Acid Sequence,
pubmed-meshheading:8598913-Animals,
pubmed-meshheading:8598913-Base Sequence,
pubmed-meshheading:8598913-Cattle,
pubmed-meshheading:8598913-Chromatography, Affinity,
pubmed-meshheading:8598913-DNA, Complementary,
pubmed-meshheading:8598913-Fungal Proteins,
pubmed-meshheading:8598913-Genetic Complementation Test,
pubmed-meshheading:8598913-HeLa Cells,
pubmed-meshheading:8598913-Humans,
pubmed-meshheading:8598913-Mediator Complex,
pubmed-meshheading:8598913-Molecular Sequence Data,
pubmed-meshheading:8598913-RNA Polymerase II,
pubmed-meshheading:8598913-Saccharomyces cerevisiae,
pubmed-meshheading:8598913-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8598913-Sequence Homology, Amino Acid,
pubmed-meshheading:8598913-TATA-Binding Protein Associated Factors,
pubmed-meshheading:8598913-Transcription, Genetic,
pubmed-meshheading:8598913-Transcription Factor TFIID,
pubmed-meshheading:8598913-Transcription Factor TFIIH,
pubmed-meshheading:8598913-Transcription Factors,
pubmed-meshheading:8598913-Transcription Factors, TFII
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pubmed:year |
1996
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pubmed:articleTitle |
A mammalian SRB protein associated with an RNA polymerase II holoenzyme.
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pubmed:affiliation |
Whitehead institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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