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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-4-19
|
| pubmed:abstractText |
Stromal cells are important regulators of mammary carcinoma growth and metastasis. We have previously shown that a 3T3-L1 adipocyte cell line secretes hepatocyte growth factor (HGF), which stimulates proliferation of a murine mammary carcinoma (SP1) in monolayer cultures (DNA Cell Biol. 13, 1189-1897, 1994). We now examine the role of growth factors and the extracellular matrix protein fibronectin in stimulation of anchorage-independent growth of SP1 cells. Purified transforming growth factor-beta (TGF-beta) stimulated significant colony growth in soft agar cultures, whereas HGF had a lesser effect. Analysis by confocal microscopy revealed that carcinoma cell colonies contained extracellular microfibrils composed of fibronectin. Partial depletion of fibronectin from 7% FBS/agar cultures reduced the number of colonies; colony growth could be recovered by adding back exogenous fibronectin. Addition of the 70-kDa N-terminal fragment of fibronectin, which inhibits fibronectin fibril formation, reduced growth of SP1 cell colonies, but an 85-kDa fragment containing the cell binding domain did not inhibit colony growth. These findings indicate that deposition of extracellular fibronectin fibrils is necessary, but not sufficient, for anchorage-independent growth of SP1 mammary carcinoma cells; growth factors are also required. SP1 cells had less fibronectin mRNA and secreted less fibronectin protein under anchorage-independent conditions than under anchorage-dependent conditions, as determined by Northern blotting and immunoprecipitation analysis. Thus, both growth factors (HGF and TGF-beta) and fibronectin may be important regulators of paracrine stimulation by stromal cells of anchorage-independent growth of mammary carcinoma cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
222
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
360-9
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8598224-3T3 Cells,
pubmed-meshheading:8598224-Adenocarcinoma,
pubmed-meshheading:8598224-Adipocytes,
pubmed-meshheading:8598224-Animals,
pubmed-meshheading:8598224-Blotting, Northern,
pubmed-meshheading:8598224-Cell Adhesion,
pubmed-meshheading:8598224-Cell Division,
pubmed-meshheading:8598224-Extracellular Matrix,
pubmed-meshheading:8598224-Female,
pubmed-meshheading:8598224-Fibronectins,
pubmed-meshheading:8598224-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:8598224-Hepatocyte Growth Factor,
pubmed-meshheading:8598224-Mammary Neoplasms, Experimental,
pubmed-meshheading:8598224-Mice,
pubmed-meshheading:8598224-Mice, Inbred CBA,
pubmed-meshheading:8598224-Neoplastic Stem Cells,
pubmed-meshheading:8598224-RNA, Messenger,
pubmed-meshheading:8598224-Stromal Cells,
pubmed-meshheading:8598224-Transforming Growth Factor beta
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Fibronectin fibrils and growth factors stimulate anchorage-independent growth of a murine mammary carcinoma.
|
| pubmed:affiliation |
Cancer Research Laboratory, Queen's University, Kingston, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|