Linked Life Data

8596728

Source:http://linkedlifedata.com/resource/pubmed/id/8596728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1996-4-18
pubmed:abstractText
The pharmacological and biophysical properties of large-conductance Ca-activated K (BK) channels from embryonic rat telencephalic neuroepithelium were investigated with in situ patch-clamp techniques. A fraction of these channels exhibited properties characteristic of BK channels recorded in well differentiated cells, including normal gating mode (BKN channels). The vast majority of BK channels expressed distinctive properties, the most conspicuous being their buzz gating mode (BKB channels). BKB channels were insensitive to a concentration of charybdotoxin that completely and reversibly blocked BKN channels. In contrast with the strict dependence of BKN channel activation on cytoplasmic Ca, BKB channels displayed substantially high open probability (Po) after inside-out patch excision in a Ca-free medium. Intracellular trypsin down-regulated the Po of BKB channels, which then exhibited a greater sensitivity to cytoplasmic Ca, mainly in the positive direction (increased Po with increased Ca). This suggested a modulatory role for Ca as opposed to its gating role in BKN channels. Ca ions also reduced current amplitude of both types of channels. BKB channels were less voltage sensitive than BKN channels, but this was not correlated with their lower Ca sensitivity. We speculate that BKB channels may represent immature forms in the developmental expression of BK channels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
431
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
763-70
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Immature properties of large-conductance calcium-activated potassium channels in rat neuroepithelium.
pubmed:affiliation
Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Building 36, Room 2CO2, 36 Convent Dr. MSC 4066, Bethesda MD 20892-4066, USA.
pubmed:publicationType
Journal Article