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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-4-17
|
| pubmed:abstractText |
Contact of mononuclear human leukocytes with cellulose dialysis membranes may result in complement-independent cell activation, i.e. enhanced synthesis of cytokines, prostaglandins and an increase in beta 2-micro-globulin synthesis. Cellular contact activation is specifically inhibited by the monosaccharide L-fucose suggesting that dialysis membrane associated L-fucose residues are involved in leukocyte activation. In this study we have detected and quantitated L-fucose on commercially-available cellulose dialysis membranes using two approaches. A sensitive enzymatic fluorescence assay detected L-fucose after acid hydrolysis of flat sheet membranes. Values ranged from 79.3 +/- 3.6 to 90.2 +/- 5.0 pmol cm-2 for Hemophan or Cuprophan respectively. Enzymatic cleavage of terminal alpha-L-fucopyranoses with alpha-L-fucosidase yielded 7.7 +/- 3.3 pmol L-fucose per cm2 for Cuprophan. Enzymatic hydrolysis of the synthetic polymer membranes AN-69 and PC-PE did not yield detectable amounts of L-fucose. In a second approach, binding of the fucose specific lectins of Lotus tetragonolobus and Ulex europaeus (UEAI) demonstrated the presence of biologically accessible L-fucose on the surface of cellulose membranes. Specific binding was observed with Cuprophan, and up to 2.6 +/- 0.3 pmol L-fucose per cm2 was calculated to be present from Langmuir-type adsorption isotherms. The data presented are in line with the hypothesis that surface-associated L-fucose residues on cellulose dialysis membranes participate in leukocyte contact activation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AN-69,
http://linkedlifedata.com/resource/pubmed/chemical/Acrylic Resins,
http://linkedlifedata.com/resource/pubmed/chemical/Acrylonitrile,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Cellulose,
http://linkedlifedata.com/resource/pubmed/chemical/Fucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hemophan,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/cuprammonium cellulose,
http://linkedlifedata.com/resource/pubmed/chemical/fucose-binding lectin,
http://linkedlifedata.com/resource/pubmed/chemical/polycarbonate-polyether copolymer
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0282-0080
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
632-8
|
| pubmed:dateRevised |
2008-8-19
|
| pubmed:meshHeading |
pubmed-meshheading:8595253-Acrylic Resins,
pubmed-meshheading:8595253-Acrylonitrile,
pubmed-meshheading:8595253-Adsorption,
pubmed-meshheading:8595253-Biocompatible Materials,
pubmed-meshheading:8595253-Cellulose,
pubmed-meshheading:8595253-Dialysis,
pubmed-meshheading:8595253-Fucose,
pubmed-meshheading:8595253-Humans,
pubmed-meshheading:8595253-Hydrolysis,
pubmed-meshheading:8595253-Kinetics,
pubmed-meshheading:8595253-Lectins,
pubmed-meshheading:8595253-Membranes, Artificial,
pubmed-meshheading:8595253-Neutrophils,
pubmed-meshheading:8595253-Phytohemagglutinins,
pubmed-meshheading:8595253-Polymers
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
L-fucose residues on cellulose-based dialysis membranes: quantification of membrane-associated L-fucose and analysis of specific lectin binding.
|
| pubmed:affiliation |
Institute of Immunology, Heidelberg, Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|