Linked Life Data

8591911

Source:http://linkedlifedata.com/resource/pubmed/id/8591911

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-4-4
pubmed:abstractText
Polymorphonuclear leukocytes (PMNs) invade the cornea following an alkali burn apparently undergoing a respiratory burst and degranulation, which is thought to lead to corneal ulceration. The supernatant obtained from burned Sigma collagen (Miller type 1) or from bovine cornea produced a significant locomotory stimulus to PMNs. Citrate inhibited this locomotory stimulus by 69.5% and 98%, respectively. PMNs were stimulated to undergo a respiratory burst without the concomitant release of beta-glucuronidase when exposed to the supernatant from alkali-burned commercial collagens, or from bovine or porcine corneas. This stimulation is reduced by 72% (Sigma collagen) or 89% (bovine cornea) when the supernatant is dialyzed against distilled water and reinstated when the osmolality is increased. The degree of the respiratory burst is partially dependent on the volume of the supernatant, the duration of alkali exposure, and/or the concentration of NaOH used. The respiratory burst of PMNs stimulated by alkali-burned Sigma collagen supernatant is inhibited by trifluoperazine but not by citrate or EDTA. Light and electron microscopy of these stimulated PMNs show many large blebs and hairlike projections. The authors hypothesize that collagen breakdown product(s) from alkali burning might be the initial, or one of the initial stimuli, for PMN invasion into the cornea and the subsequent activation of the respiratory burst.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-304
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Alkali-burned collagen produces a locomotory and metabolic stimulant to neutrophils.
pubmed:affiliation
Eye Research Laboratories, Brookwood Medical Center, Birmingham, Alabama, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't