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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-3-28
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pubmed:abstractText |
Most plant mitochondrial genomes exist as subgenomic-size fragments apparently due to recombination between repetitive sequences. This leads to the possibility that independently replicating subgenomic domains could result in mitochondrial gene copy number variation. We show, through Southern-blot analysis of both restricted and intact mtDNA, that there are gene-specific copy number differences in the monocot Zea mays. Comparison of two different maize genotypes, B37(N) and B37(T), a cytoplasmic male-sterile strain, reveal fewer gene copy number differences for B37(T) than for B37(N). In contrast to maize, significant gene copy number differences are not detected in the dicot Brassica hirta. We also demonstrate that mitochondrial transcriptional rates in both species are apparently dependent on gene copy number since relative rates determined by run-on analysis are proportional to relative gene copy numbers. Thus a direct relationship exists between plant mitochondrial gene copy number and transcriptional rate.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0172-8083
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
113-21
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pubmed:dateRevised |
2002-11-1
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pubmed:meshHeading |
pubmed-meshheading:8590461-Blotting, Southern,
pubmed-meshheading:8590461-DNA, Mitochondrial,
pubmed-meshheading:8590461-DNA, Plant,
pubmed-meshheading:8590461-Gene Amplification,
pubmed-meshheading:8590461-Gene Expression Regulation, Plant,
pubmed-meshheading:8590461-Mitochondria,
pubmed-meshheading:8590461-RNA, Messenger,
pubmed-meshheading:8590461-Restriction Mapping,
pubmed-meshheading:8590461-Transcription, Genetic,
pubmed-meshheading:8590461-Zea mays
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pubmed:year |
1995
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pubmed:articleTitle |
Selective DNA amplification regulates transcript levels in plant mitochondria.
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pubmed:affiliation |
Department of Immunology and Medical Microbiology, College of Medicine, University of Florida, Gainesville 32610, USA.
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pubmed:publicationType |
Journal Article
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