Linked Life Data

8589845

Source:http://linkedlifedata.com/resource/pubmed/id/8589845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-3-27
pubmed:abstractText
The first extracellular domain of the alpha-subunit of the Na+/K+-ATPase (sodium/potassium pump) is functionally important, affecting sensitivity of the enzyme to cardiac glycosides (e.g. ouabain) and being implicated in the transport of K+. This domain is also variable among mammalian isoforms of the alpha-subunit. Using PCR, we have isolated from seven insect species with contrasting physiologies a DNA fragment containing this region, in order to help determine whether tissue-specific expression might be associated with isoforms encoded by a gene family, as it is in mammals. A single sequence (with one ORF) characteristic of Na+/K+-ATPase was obtained from genomic DNA of each species. Only the fragment from Manduca sexta contained an intron, but at a location different to that found in mammals. For all Diptera so far characterized, the species phylogeny is the same as the alpha-subunit gene phylogeny (based on the sequences of the first extracellular domain and flanking transmembrane domains). The results strongly indicate a single, ouabain-sensitive isoform of the alpha-subunit of Na+/K+-ATPase is present in Diptera.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0962-1075
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-92
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A single isoform of the Na+/K(+)-ATPase alpha-subunit in Diptera: evidence from characterization of the first extracellular domain.
pubmed:affiliation
Department of Entomology, Natural History Museum, London.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't