Linked Life Data

8589348

Source:http://linkedlifedata.com/resource/pubmed/id/8589348

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-3-25
pubmed:abstractText
The metabolic changes in a rat hepatoma cell line, AH70 cells, after co-culture with rat Kupffer cells (KC) were visualized and analysed using a fluorescence microscope equipped with a silicon intensified target camera and a laser scanning confocal microscopic system. Kupffer cells were isolated from male Wistar rats, and cultured without any stimuli. The non-activated KC reduced the mitochondrial energization in the cocultured AH70 cells within 2 h, which was indicated by decreased rhodamine 123 (Rh123) fluorescence. Either NG-monomethyl-L-arginine or dexamethasone significantly attenuated the KC-induced mitochondrial dysfunction in AH70 cells, suggesting the involvement of nitric oxide (NO) derived from inducible-type nitric oxide synthase (iNOS). Administration of monoclonal antibody (mAb) directed against rat ICAM-1 also prevented the decrease in Rh123 fluorescence. Electron microscopy revealed that the membrane-to-membrane attachment between KC and AH70 cells occurred within 2 h. A laser scanning confocal microscopic observation using mAb against ICAM-1 presented that the ICAM-1 expression on AH70 cells and KC increased after the co-culture. It is therefore concluded that the KC-mediated mitochondrial dysfunction of hepatoma cells largely depends on NO production by iNOS. Furthermore, the present study supports a scenario that the NO production and release from KC is triggered by the close contact with hepatoma cells through adhesion molecules such as ICAM-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0815-9319
pubmed:author
pubmed:issnType
Print
pubmed:volume
10 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S68-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8589348-Animals, pubmed-meshheading:8589348-Antibodies, Monoclonal, pubmed-meshheading:8589348-Arginine, pubmed-meshheading:8589348-Cell Adhesion, pubmed-meshheading:8589348-Coculture Techniques, pubmed-meshheading:8589348-Dexamethasone, pubmed-meshheading:8589348-Fluorescent Dyes, pubmed-meshheading:8589348-Intercellular Adhesion Molecule-1, pubmed-meshheading:8589348-Kupffer Cells, pubmed-meshheading:8589348-Liver Neoplasms, Experimental, pubmed-meshheading:8589348-Male, pubmed-meshheading:8589348-Microscopy, Fluorescence, pubmed-meshheading:8589348-Mitochondria, Liver, pubmed-meshheading:8589348-Nitric Oxide, pubmed-meshheading:8589348-Nitric Oxide Synthase, pubmed-meshheading:8589348-Rats, pubmed-meshheading:8589348-Rats, Wistar, pubmed-meshheading:8589348-Rhodamine 123, pubmed-meshheading:8589348-Rhodamines, pubmed-meshheading:8589348-Tumor Cells, Cultured, pubmed-meshheading:8589348-omega-N-Methylarginine
pubmed:year
1995
pubmed:articleTitle
Nitric oxide mediates mitochondrial dysfunction in hepatoma cells induced by non-activated Kupffer cells: evidence implicating ICAM-1-dependent process.
pubmed:affiliation
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't