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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
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|
pubmed:dateCreated |
1996-3-22
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|
pubmed:abstractText |
To improve Salmonella detection, we developed nonradioactive hybridization assays of amplified products from pure Salmonella cultures. Biotin-labeled PCR products were trapped by internal probes covalently bound to CovaLink-NH MicroWells and detected by colorimetric or chemiluminescent enzymatic reactions. The sensitivities of colorimetric assays using peroxidase and alkaline phosphatase were similar to those obtained with an ethidium bromide-stained agarose gel; both procedures allow the detection of 50 Salmonella cells. Chemiluminescence was 10-fold more sensitive than colorimetry.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0736-6205
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
792-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8588918-Alkaline Phosphatase,
pubmed-meshheading:8588918-Colorimetry,
pubmed-meshheading:8588918-Coloring Agents,
pubmed-meshheading:8588918-DNA, Bacterial,
pubmed-meshheading:8588918-Electrophoresis, Agar Gel,
pubmed-meshheading:8588918-Ethidium,
pubmed-meshheading:8588918-Luminescent Measurements,
pubmed-meshheading:8588918-Nucleic Acid Hybridization,
pubmed-meshheading:8588918-Peroxidase,
pubmed-meshheading:8588918-Polymerase Chain Reaction,
pubmed-meshheading:8588918-Salmonella,
pubmed-meshheading:8588918-Sensitivity and Specificity
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|
pubmed:year |
1995
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|
pubmed:articleTitle |
Chemiluminescent and colorimetric enzymatic assays for the detection of PCR-amplified Salmonella sp. products in microplates.
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|
pubmed:affiliation |
Centre National d'Etudes Veterinaires et Alimentaires Ploufragan, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
