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pubmed-article:8588089pubmed:abstractTextMonoclonal antibodies to sheep light chain, IgM and IgG were produced and used to assess total immunoglobulin (Ig) synthesis by sheep B cells in culture and antibodies to specific antigens. By using these antibodies in a dual fluorescence-activated cell sorting analysis of sheep efferent lymph B lymphocytes the percentage change in surface Ig isotype of B lymphoblasts from IgM to IgG after the antigenic stimulation of the local lymph node was measured. An extension of this analysis to paired blood and afferent or efferent lymph B cells made it possible to investigate the recirculation characteristics of B cells expressing different Ig isotypes.lld:pubmed
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pubmed-article:8588089pubmed:articleTitleAnalysis of the expression and secretion of isotypes of sheep B cell immunoglobulins with a panel of isotype-specific monoclonal antibodies.lld:pubmed
pubmed-article:8588089pubmed:affiliationDepartment of Veterinary Pathology, University of Edinburgh, Summerhall.lld:pubmed
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