rdf:type |
|
lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0004561,
umls-concept:C0017262,
umls-concept:C0021017,
umls-concept:C0021027,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0036945,
umls-concept:C0185117,
umls-concept:C0441833,
umls-concept:C0936012,
umls-concept:C1999270,
umls-concept:C2911684
|
pubmed:issue |
3
|
pubmed:dateCreated |
1996-3-28
|
pubmed:abstractText |
Monoclonal antibodies to sheep light chain, IgM and IgG were produced and used to assess total immunoglobulin (Ig) synthesis by sheep B cells in culture and antibodies to specific antigens. By using these antibodies in a dual fluorescence-activated cell sorting analysis of sheep efferent lymph B lymphocytes the percentage change in surface Ig isotype of B lymphoblasts from IgM to IgG after the antigenic stimulation of the local lymph node was measured. An extension of this analysis to paired blood and afferent or efferent lymph B cells made it possible to investigate the recirculation characteristics of B cells expressing different Ig isotypes.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0034-5288
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
59
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
189-94
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8588089-Animals,
pubmed-meshheading:8588089-Antibodies, Monoclonal,
pubmed-meshheading:8588089-Antibody Specificity,
pubmed-meshheading:8588089-B-Lymphocytes,
pubmed-meshheading:8588089-Cells, Cultured,
pubmed-meshheading:8588089-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8588089-Flow Cytometry,
pubmed-meshheading:8588089-Immunoelectrophoresis,
pubmed-meshheading:8588089-Immunoglobulin G,
pubmed-meshheading:8588089-Immunoglobulin Isotypes,
pubmed-meshheading:8588089-Immunoglobulin Light Chains,
pubmed-meshheading:8588089-Immunoglobulin M,
pubmed-meshheading:8588089-Lymph Nodes,
pubmed-meshheading:8588089-Sheep
|
pubmed:year |
1995
|
pubmed:articleTitle |
Analysis of the expression and secretion of isotypes of sheep B cell immunoglobulins with a panel of isotype-specific monoclonal antibodies.
|
pubmed:affiliation |
Department of Veterinary Pathology, University of Edinburgh, Summerhall.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|