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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1996-3-27
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pubmed:abstractText |
We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified ORF-2 protein showed a single polypeptide of 75 kDa by Coomassie blue staining and by Western blot (immunoblot) analysis. We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-13438536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-1373282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-1448913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-1588318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-1630924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-1731327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-1926770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-2106181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-2864544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-2939734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-3099621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-3115739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-3116288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-6107544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-6409836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-6412542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-6427404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-6438353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-6770682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-8335973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8586723-8370746
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3308-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8586723-Animals,
pubmed-meshheading:8586723-Cell Line,
pubmed-meshheading:8586723-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8586723-Gene Expression,
pubmed-meshheading:8586723-Genetic Vectors,
pubmed-meshheading:8586723-Hepatitis Antibodies,
pubmed-meshheading:8586723-Hepatitis E,
pubmed-meshheading:8586723-Hepatitis E virus,
pubmed-meshheading:8586723-Humans,
pubmed-meshheading:8586723-Immunoglobulin M,
pubmed-meshheading:8586723-Nucleopolyhedrovirus,
pubmed-meshheading:8586723-Open Reading Frames,
pubmed-meshheading:8586723-Protein Folding,
pubmed-meshheading:8586723-Recombinant Proteins,
pubmed-meshheading:8586723-Spodoptera,
pubmed-meshheading:8586723-Viral Structural Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked immunosorbent assay.
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pubmed:affiliation |
Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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