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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-3-25
|
| pubmed:abstractText |
We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted into liposomes comprising Salmonella minnesota R595 lipopolysaccharides, and fusion of spleen cells with a mouse myeloma cell line. One monoclonal antibody, SHS-1, which was generated by immunizing mice with purified i-active ganglioside(NeuGc), reacted specifically with the i-active ganglioside(NeuGc) used as an immunogen. Structurally related gangliosides, such as GM3(NeuGc), sialosylparagloboside (SPG) (NeuGc), or I-active ganglioside(NeuGc), corresponding gangliosides [GM3 containing N-acetylneuraminic acid (NeuAc), SPG(NeuAc), i-active ganglioside(NeuAc), and I-active ganglioside(NeuAc)], other gangliosides, or neutral glycosphingolipid (GSL) were not recognized by the monoclonal antibody. These findings indicate that the SHS-1 monoclonal antibody may be specific for NeuGc-containing i-active ganglioside. On the other hand, the other two monoclonal antibodies, MSG-1 and SPS-20, which were generated by immunizing mice with purified ganglioside GM3(NeuGc) and SPG(NeuGc), respectively, showed cross-reactivity to structurally related gangliosides. The MSG-1 monoclonal antibody exhibited reactivity to ganglioside GM3(NeuAc). The SPS-20 monoclonal antibody also cross-reacted with SPG(NeuAc), i-active ganglioside(NeuGc), and i-active ganglioside(NeuAc). Neither MSG-1 nor SPS-20 reacted with corresponding gangliosides, other gangliosides, or neutral GSLs tested. Using the SHS-1 antibody specific for i-active ganglioside(NeuGc), we studied the expression of NeuGc-containing antigen in human colon cancer tissue. An NeuGc-containing glycoconjugate was detected in the colon cancer tissue.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Heterophile,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Heterophile,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Gangliosides,
http://linkedlifedata.com/resource/pubmed/chemical/Globosides,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/sialosylparagloboside
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-924X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
117
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1062-9
|
| pubmed:dateRevised |
2007-12-19
|
| pubmed:meshHeading |
pubmed-meshheading:8586620-Animals,
pubmed-meshheading:8586620-Antibodies, Heterophile,
pubmed-meshheading:8586620-Antibodies, Monoclonal,
pubmed-meshheading:8586620-Antibody Formation,
pubmed-meshheading:8586620-Antibody Specificity,
pubmed-meshheading:8586620-Antigens, Heterophile,
pubmed-meshheading:8586620-Antigens, Neoplasm,
pubmed-meshheading:8586620-Carbohydrate Sequence,
pubmed-meshheading:8586620-Colonic Neoplasms,
pubmed-meshheading:8586620-Gangliosides,
pubmed-meshheading:8586620-Globosides,
pubmed-meshheading:8586620-Humans,
pubmed-meshheading:8586620-Immunohistochemistry,
pubmed-meshheading:8586620-Liposomes,
pubmed-meshheading:8586620-Mice,
pubmed-meshheading:8586620-Mice, Inbred BALB C,
pubmed-meshheading:8586620-Molecular Sequence Data,
pubmed-meshheading:8586620-Neuraminic Acids
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Production of monoclonal antibodies directed to Hanganutziu-Deicher active gangliosides, N-glycolylneuraminic acid-containing gangliosides.
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| pubmed:affiliation |
Department of Cell Chemistry, Okayama University Medical School.
|
| pubmed:publicationType |
Journal Article
|