Linked Life Data

8586422

Source:http://linkedlifedata.com/resource/pubmed/id/8586422

Statements in which the resource exists.
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pubmed-article:8586422pubmed:abstractTextWe have mapped a region of high loss of heterozygosity in breast cancer to a 2-cM interval between the loci D1S430 and D1S465 on chromosome 1p31.1. This region shows allelic imbalance in around 60% of breast tumors. As part of a strategy to clone the target gene(s) within this interval, we have generated a yeast artificial chromosome contig spanning over 7 Mb. YACs from the CEPH and Zeneca (formerly ICI) libraries have been obtained by screening with PCR-based STSs from the region for both previously identified loci and newly isolated STSs. The YACs have been assembled into a contig by a combination of approaches, including analysis of their STS content, generation of new STSs from the ends of key YACs, and long-range restriction mapping. These YAC clones provide the basis for complete characterization of the region of high loss in breast cancer and for the ultimate identification of the target gene(s).lld:pubmed
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pubmed-article:8586422pubmed:articleTitleIdentification and cloning in yeast artificial chromosomes of a region of elevated loss of heterozygosity on chromosome 1p31.1 in human breast cancer.lld:pubmed
pubmed-article:8586422pubmed:affiliationCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Manchester, United Kingdom.lld:pubmed
pubmed-article:8586422pubmed:publicationTypeJournal Articlelld:pubmed