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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1996-3-22
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pubmed:abstractText |
We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0173-0835
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1977-81
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:8586073-Animals,
pubmed-meshheading:8586073-Chromatography, High Pressure Liquid,
pubmed-meshheading:8586073-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:8586073-Hydrogen-Ion Concentration,
pubmed-meshheading:8586073-Information Systems,
pubmed-meshheading:8586073-Isoelectric Point,
pubmed-meshheading:8586073-Liver,
pubmed-meshheading:8586073-Mice,
pubmed-meshheading:8586073-Molecular Weight,
pubmed-meshheading:8586073-Peptide Fragments,
pubmed-meshheading:8586073-Proteins,
pubmed-meshheading:8586073-Rats,
pubmed-meshheading:8586073-Sequence Analysis,
pubmed-meshheading:8586073-Trypsin
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pubmed:year |
1995
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pubmed:articleTitle |
An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies.
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pubmed:affiliation |
Large Scale Biology Corporation, Rockville, MD 20850-3338, USA. leigh@lsbc,com
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pubmed:publicationType |
Journal Article
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