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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1996-3-19
|
| pubmed:abstractText |
Cytotoxic T lymphocytes (CTL) play a major role in protective immunity against viral diseases. However, the antigenic formulations that can be used in vaccinations able to generate virus-specific CTL responses in vivo have yet to be defined. We have previously shown that HIV-1-specific CTL can be elicited in mice by injecting without adjuvant a synthetic peptide of the envelope glycoprotein that has been modified by the addition of a simple lipid tail to the end of the sequence (lipopeptide). The present study set out to address the question of whether such immunogens may be appropriate for preparing a human synthetic vaccine. We first showed that CTL were effectively induced by lipopeptides when given s.c. or i.p. We evidenced that the in vivo induction required stimulation of a concomitant specific T helper cell response, implying the presence of at least one CD4 epitope in the synthetic sequence used. Bearing in mind the particular properties that would be required in a prospective human peptide vaccine, we conceived a strategy in which a virus-specific CTL response could be generated in mice of different haplotypes using a single lipopeptide. We therefore tested a lipopeptide construct that integrated a synthetic sequence having three colinear epitopes of the influenza virus nucleoprotein, each restricted to a different H-2 haplotype. We found that a CTL response could be elicited to all three epitopes of this chimeric multirestricted lipopeptide construct. Finally, we have attempted to estimate the duration of the responses; strong CTL activities were still present up to six months after the last injection. These findings indicate that this approach may be suitable for developing a synthetic vaccine for human use.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0264-410X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1339-45
|
| pubmed:dateRevised |
2005-11-22
|
| pubmed:meshHeading |
pubmed-meshheading:8585291-Animals,
pubmed-meshheading:8585291-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8585291-Chick Embryo,
pubmed-meshheading:8585291-Epitopes,
pubmed-meshheading:8585291-Haplotypes,
pubmed-meshheading:8585291-Influenza A virus,
pubmed-meshheading:8585291-Influenza B virus,
pubmed-meshheading:8585291-Influenza Vaccines,
pubmed-meshheading:8585291-Lipoproteins,
pubmed-meshheading:8585291-Mice,
pubmed-meshheading:8585291-Mice, Inbred Strains,
pubmed-meshheading:8585291-Nucleoproteins,
pubmed-meshheading:8585291-Recombinant Fusion Proteins,
pubmed-meshheading:8585291-Sensitivity and Specificity,
pubmed-meshheading:8585291-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8585291-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:8585291-Time Factors,
pubmed-meshheading:8585291-Viral Proteins
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Long-lasting anti-viral cytotoxic T lymphocytes induced in vivo with chimeric-multirestricted lipopeptides.
|
| pubmed:affiliation |
Laboratoire d'Immunologie des Intéractions Cellulaires et Moléculaires, INSERM U152, Institut Cochin de Génétique Moléculaire, Paris, France.
|
| pubmed:publicationType |
Journal Article
|