Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-3-18
pubmed:abstractText
N-methyl-D-aspartate (NMDA), a specific agonist of the NMDA-type glutamate receptor, has been shown to stimulate the release of several anterior pituitary hormones when administered in vivo. The primary site of action of NMDA has been suggested to be at the level of the hypothalamus via the control of hypothalamic releasing factors. However, recent studies have demonstrated that NMDA can exert stimulatory effects directly upon anterior pituitary cells perifused in vitro, suggesting that the anterior pituitary may also be a site of action for glutamate. Hence, the purpose of the present study was to determine whether the NMDA R1 receptor subunit is co-localized in specific hormone-secreting cells of the anterior pituitary. To achieve this aim, immunohistochemical studies were performed using an antibody specific for the NMDA R1 receptor subunit to stain sections from the pituitaries of steroid-treated ovariectomized immature rats. Double-immunohistochemistry was employed to demonstrate co-localization. The results of the studies revealed that moderate immunoreactive NMDA R1 receptor staining was observed in the anterior and intermediate lobe of the pituitary with little staining observed in the posterior lobe. Double-immunohistochemistry using antibodies for the NMDA R1 receptor subunit and specific anterior pituitary hormones revealed that the NMDA R1 receptor subunit is co-localized in many cell types of the anterior pituitary including LH (11.2%), FSH (9%), GH (4.6%), TSH (5.7%) and PRL cells (8%). Only one ACTH cell was found to co-localize the NMDA R1 receptor subunit.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0028-3835
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
178-86
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8584117-Amino Acid Sequence, pubmed-meshheading:8584117-Animals, pubmed-meshheading:8584117-Blotting, Northern, pubmed-meshheading:8584117-DNA Probes, pubmed-meshheading:8584117-Female, pubmed-meshheading:8584117-Follicle Stimulating Hormone, pubmed-meshheading:8584117-Growth Hormone, pubmed-meshheading:8584117-Immunohistochemistry, pubmed-meshheading:8584117-Luteinizing Hormone, pubmed-meshheading:8584117-Molecular Sequence Data, pubmed-meshheading:8584117-N-Methylaspartate, pubmed-meshheading:8584117-Nucleic Acid Hybridization, pubmed-meshheading:8584117-Ovariectomy, pubmed-meshheading:8584117-Pituitary Gland, Anterior, pubmed-meshheading:8584117-Prolactin, pubmed-meshheading:8584117-RNA, Messenger, pubmed-meshheading:8584117-Rats, pubmed-meshheading:8584117-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:8584117-Thyrotropin
pubmed:year
1995
pubmed:articleTitle
Localization of the N-methyl-D-aspartate R1 receptor subunit in specific anterior pituitary hormone cell types of the female rat.
pubmed:affiliation
Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912-3000, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.