Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-3-20
pubmed:abstractText
P elements are widely used as insertional mutagens to tag genes, facilitating molecular cloning and analyses. We modified a P element so that it carried two copies of the suppressor of Hairy-wing [su(Hw)] binding regions isolated from the gypsy transposable element. This transposon was mobilized, and the genetic consequences of its insertion were analyzed. Gene expression can be altered by the su(Hw) protein as a result of blocking the interaction between enhancer/silencer elements and their promoter. These effects can occur over long distances and are general. Therefore, a composite transposon (SUPor-P for suppressor-P element) combines the mutagenic efficacy of the gypsy element with the controllable transposition of P elements. We show that, compared to standard P elements, this composite transposon causes an expanded repertoire of mutations and produces alleles that are suppressed by su(Hw) mutations. The large number of heterochromatic insertions obtained is unusual compared to other insertional mutagenesis procedures, indicating that the SUPor-P transposon may be useful for studying the structural and functional properties of heterochromatin.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1311855, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1327958, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1334894, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1375573, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1545824, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1821846, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1849859, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1851585, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-1900919, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2087785, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2111263, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2558049, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2558050, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2820057, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2829026, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2830671, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2835286, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2842772, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-2853469, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-3023068, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-3037327, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-3096713, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-3123324, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-3146523, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-6295640, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-6300868, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-6309410, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-6319027, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-7758950, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-8032206, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-8382177, http://linkedlifedata.com/resource/pubmed/commentcorrection/8582613-8382607
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1061-74
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:8582613-Animals, pubmed-meshheading:8582613-Chromosomes, pubmed-meshheading:8582613-DNA, Recombinant, pubmed-meshheading:8582613-DNA Transposable Elements, pubmed-meshheading:8582613-DNA-Binding Proteins, pubmed-meshheading:8582613-Drosophila Proteins, pubmed-meshheading:8582613-Drosophila melanogaster, pubmed-meshheading:8582613-Female, pubmed-meshheading:8582613-Gene Expression Regulation, pubmed-meshheading:8582613-Genes, Insect, pubmed-meshheading:8582613-Genes, Suppressor, pubmed-meshheading:8582613-Genetic Complementation Test, pubmed-meshheading:8582613-Genetic Markers, pubmed-meshheading:8582613-Male, pubmed-meshheading:8582613-Mutagenesis, Insertional, pubmed-meshheading:8582613-Nuclear Proteins, pubmed-meshheading:8582613-Repressor Proteins, pubmed-meshheading:8582613-Retroelements
pubmed:year
1995
pubmed:articleTitle
A P element containing suppressor of hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster.
pubmed:affiliation
Department of Biochemistry, University of Iowa, Iowa City 52242, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.