Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1996-3-20
|
| pubmed:databankReference | |
| pubmed:abstractText |
A 107-kD protein has been identified in primary cultures of chicken embryonic cardiomyocytes by immunoprecipitations with certain anti-nebulin monoclonal antibodies (mAbs). These mAbs, prepared against a fragment of human skeletal muscle nebulin located near the carboxyl terminus, detect a 107-kD protein in extracts of adult chicken heart, adult mouse heart, and adult rabbit heart by immunoblot analysis. A partial cDNA corresponding to this protein has been isolated by immunological screening of a chicken heart cDNA expression vector library. The partial cDNA encodes a 380-amino acid open reading frame composed entirely of nebulin-like 35-residue modules marked by the highly conserved sequence motifs: SXXXYK and TPD. The open reading frame exhibits 60-85% homology with skeletal muscle nebulins from a variety of species. This cDNA recognizes an approximately 8-kb transcript in cardiac RNA and does not hybridize to skeletal muscle RNAs by northern analysis. Immunofluorescence localization of this nebulin-like protein in primary cultures of chicken cardiomyocytes and embryonic chicken cardiac myofibrils indicates that the protein is localized to the I-Z-I complex of the myofibrils, extending approximately 25% of the thin filament length. Comparisons of the distribution of this protein relative to actin, myosin, and titin in spreading cardiomyocytes suggest that the cardiac nebulin-like protein becomes aligned with the nascent myofibrils early during myofibrillogenesis. To distinguish this petite nebulin-like protein from the 600-900 kD skeletal muscle nebulin, we have named it nebulette.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/LIM Domain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/nebulin
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0886-1544
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
205-25
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8581976-Animals,
pubmed-meshheading:8581976-Antibodies, Monoclonal,
pubmed-meshheading:8581976-Antibody Specificity,
pubmed-meshheading:8581976-Base Sequence,
pubmed-meshheading:8581976-Carrier Proteins,
pubmed-meshheading:8581976-Cells, Cultured,
pubmed-meshheading:8581976-Chick Embryo,
pubmed-meshheading:8581976-Cross Reactions,
pubmed-meshheading:8581976-Cytoskeletal Proteins,
pubmed-meshheading:8581976-DNA, Complementary,
pubmed-meshheading:8581976-Gene Library,
pubmed-meshheading:8581976-Genetic Testing,
pubmed-meshheading:8581976-Humans,
pubmed-meshheading:8581976-Immunoblotting,
pubmed-meshheading:8581976-LIM Domain Proteins,
pubmed-meshheading:8581976-Mice,
pubmed-meshheading:8581976-Mice, Inbred BALB C,
pubmed-meshheading:8581976-Molecular Sequence Data,
pubmed-meshheading:8581976-Molecular Weight,
pubmed-meshheading:8581976-Muscle, Skeletal,
pubmed-meshheading:8581976-Muscle Proteins,
pubmed-meshheading:8581976-Myocardium,
pubmed-meshheading:8581976-Myofibrils,
pubmed-meshheading:8581976-Rabbits,
pubmed-meshheading:8581976-Sarcomeres,
pubmed-meshheading:8581976-Sequence Analysis, DNA,
pubmed-meshheading:8581976-Sequence Homology, Amino Acid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Nebulette: a 107 kD nebulin-like protein in cardiac muscle.
|
| pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Texas, Austin 78712, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|