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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-3-19
|
| pubmed:abstractText |
The putatative effects of different estrogen levels on the expression of non-muscle myosin isoforms in rabbit myometrium have been investigated using three monoclonal anti-platelet myosin heavy chain (MyHC) antibodies (NM-F6, NM-G2, and NM-A9). Western blotting analysis of proteolytic digests of human platelet actomyosin indicates that these antibodies are specific for three distinct epitopes. Comparative immunofluorescence tests on cultered human fibroblasts with polyclonal sequence-specific anti-MyHCA antibody suggest that the patterns of NM-F6, NM-.G2 and NM-A9, although similar, do not overlap with that of type-A MyHC. Distribution of NM myosin isoforms has been studied in indirect immunofluorescence assays using cryosections of tissues from rabbits at various stages of development, pregnancy, or from ovariectomized, 17beta-estradiol-treated ovariectomized, and human chorionic gonadotropin-treated animals. Non-muscle myosin antigenicity is still present in the myometrium when the female becomes sexually competent. The immunoreactivity of non-muscle myosin for NM-F6 is steroid-independent, since it does not change with pregnancy or ovariectomy, but that of NM-G2 is estrogen-dependent; the latter disappears during pregnancy and in ovariectomized animals treated with estradiol, whereas it is expressed in ovariectomized rabbits. Although non-muscle myosin immunoreactivity for NM-A9 is detectable under all the experimental conditions, it can assume different patterns of intracellular distribution in vitro (punctate vs filamentous), depending on culture conditions and the presence of estrogens.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0302-766X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
283
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7-18
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:8581961-Animals,
pubmed-meshheading:8581961-Antibodies, Monoclonal,
pubmed-meshheading:8581961-Antibody Specificity,
pubmed-meshheading:8581961-Blotting, Western,
pubmed-meshheading:8581961-Cell Differentiation,
pubmed-meshheading:8581961-Cells, Cultured,
pubmed-meshheading:8581961-Chorionic Gonadotropin,
pubmed-meshheading:8581961-Epitope Mapping,
pubmed-meshheading:8581961-Estrogens,
pubmed-meshheading:8581961-Female,
pubmed-meshheading:8581961-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:8581961-Humans,
pubmed-meshheading:8581961-Immunohistochemistry,
pubmed-meshheading:8581961-Isomerism,
pubmed-meshheading:8581961-Muscle, Smooth,
pubmed-meshheading:8581961-Myometrium,
pubmed-meshheading:8581961-Myosins,
pubmed-meshheading:8581961-Ovariectomy,
pubmed-meshheading:8581961-Pregnancy,
pubmed-meshheading:8581961-Rabbits
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Expression of non-muscle myosin isoforms in rabbit myometrium is estrogen-dependent.
|
| pubmed:affiliation |
Department of Biomedical Sciences, University of Padua, Padua, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|