Linked Life Data

8581400

Source:http://linkedlifedata.com/resource/pubmed/id/8581400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-3-19
pubmed:abstractText
Molecular cloning has revealed the existence of four distinct transporters for the inhibitory neurotransmitter gamma-aminobutyric acid (GABA), termed GAT-1, GAT-2, GAT-3, and BGT-1. To determine which of the cloned transporters are in neurons and which are in glia, we have undertaken a combined pharmacological and molecular biological study using cell cultures derived from rat brain. In neuronal cultures approximately 70% of GABA transport is sensitive to the GAT-1-selective ligand NNC-711 and drug potencies at this site correlate well with their potencies at GAT-1; GAT-1 mRNA is abundant in these cultures as determined by northern blot analysis. Drug potencies at the NNC-711-resistant component correlate well with their potencies at GAT-2 and GAT-3, whose pharmacological profiles are similar to one another. Northern blots reveal the presence of mRNA for GAT-3 in neuronal cultures, whereas GAT-2 and BGT-1 mRNAs are not detected. Type 1 astrocyte cultures exhibit very low levels of GABA transport activity, which has very low potency for GABA but high potency for taurine. Such cultures have mRNA for a taurine transporter and BGT-1, but not for GAT-1, GAT-2, and GAT-3. In cultures containing O-2A progenitor cells and Type 2 astrocytes, approximately 75% of GABA uptake is sensitive to NNC-711 and drug potencies at this site correlate well with their potencies at GAT-1; GAT-1 mRNA is abundant. Drug potencies at the NNC-711-resistant component correlate well with their potencies at GAT-2 and GAT-3; mRNAs for both of these transporters are present (though GAT-2 mRNA is the more abundant), as is BGT-1 mRNA. In summary, these data demonstrate heterogeneity of both neuronal and glial GABA transport.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1060-6823
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-46
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Re-evaluation of GABA transport in neuronal and glial cell cultures: correlation of pharmacology and mRNA localization.
pubmed:affiliation
Department of Pharmacology, Synaptic Pharmaceutical Corporation, Paramus, NJ 07652, USA.
pubmed:publicationType
Journal Article