Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1996-3-18
|
| pubmed:abstractText |
Multiple sclerosis (MS) is a putative autoimmune disease that is linked with HLA-DR2,w15. Proteolipid protein (PLP) is a candidate autoantigen in MS, but the disease-associated epitopes have not been determined. Using overlapping and non-overlapping PLP peptides, we have studied the T cell response to the major hydrophilic domain PLP 85-159 in the peripheral blood of MS and healthy subjects (HS). Short-term T cell lines (TCL) were selected against each peptide using microwell plates and the frequency of peptide-specific TCL was estimated. PLP 95-116-specific TCL were most efficiently generated and the frequency was significantly higher in MS compared with HS (P < 0.05). When compared between DR2,w15+ and DR2,w15- MS, TCL frequency to PLP 95-116 was significantly higher in DR2,w15+ MS (P < 0.005) and TCL reactive to the overlapping peptide 105-124 were also increased in DR2,w15+ MS (P < 0.025). Using DR gene-transfected L cells, we could show that the DRB1*1501 product of the DR2 haplotype presents PLP 95-116 to TCL selected against the peptide. These results imply that PLP 95-116 represents a major epitope for the DR2,w15+ MS.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0953-8178
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1771-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8580075-Adult,
pubmed-meshheading:8580075-Amino Acid Sequence,
pubmed-meshheading:8580075-Animals,
pubmed-meshheading:8580075-Cell Line,
pubmed-meshheading:8580075-Epitopes,
pubmed-meshheading:8580075-Female,
pubmed-meshheading:8580075-HLA-DR2 Antigen,
pubmed-meshheading:8580075-Haplotypes,
pubmed-meshheading:8580075-Histocompatibility Testing,
pubmed-meshheading:8580075-Humans,
pubmed-meshheading:8580075-Lymphocyte Count,
pubmed-meshheading:8580075-Male,
pubmed-meshheading:8580075-Mice,
pubmed-meshheading:8580075-Middle Aged,
pubmed-meshheading:8580075-Molecular Sequence Data,
pubmed-meshheading:8580075-Multiple Sclerosis,
pubmed-meshheading:8580075-Myelin Proteolipid Protein,
pubmed-meshheading:8580075-Peptide Fragments,
pubmed-meshheading:8580075-T-Lymphocytes
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Analysis of proteolipid protein (PLP)-specific T cells in multiple sclerosis: identification of PLP 95-116 as an HLA-DR2,w15-associated determinant.
|
| pubmed:affiliation |
Department of Demyelinating Disease and Aging, National Institute of Neuroscience, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|