Linked Life Data

8579358

Source:http://linkedlifedata.com/resource/pubmed/id/8579358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-3-8
pubmed:abstractText
The membrane protein CD36 has been implicated in platelet and monocyte signal transduction events and is known to be tightly associated with cytosolic protein tyrosine kinases. CD36 contains an extremely small cytoplasmic domain(s) and the mechanism by which CD36 interacts with cytosolic kinases is unknown. In the present study, CD36 (M(r) 88,000) has been detected on the surface of platelets as a conformational isoform or complex of apparent M(r) 113,000. In intact platelets crosslinked with bis(sulfosuccinimidyl)suberate, approximately 50% of cell surface CD36 existed as the 113-kDa form. When detergent extracts of platelets were crosslinked, the amount of CD36 in the 113-kDa form was found to be dependent on the detergent used. The 113-kDa form of CD36 was 10-fold more prevalent in Triton X-100 extracts than in extracts made with the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS). Addition of Triton X-100 to CHAPS platelet extracts resulted in recovery of the 113-kDa form of CD36. These studies suggest that CD36 exists on the surface of platelets as a heterodimeric complex of CD36 and another protein(s) or exists in two different conformational states which, when covalently crosslinked, exhibit an apparent M(r) of 113,000. Further characterization of the 113-kDa form of CD36 may help define CD36-kinase interactions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
326
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
A detergent-sensitive 113-kDa conformer/complex of CD36 exists on the platelet surface.
pubmed:affiliation
Department of Biochemistry, Holland Laboratory, Rockville, Maryland 20855, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.