Linked Life Data

8578641

Source:http://linkedlifedata.com/resource/pubmed/id/8578641

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1996-3-13
pubmed:abstractText
Lymphocytes of intestinal epithelia were examined in lambs experimentally infected with coccidia. Fourteen conventional, coccidia-free 3-week-old lambs of the Dala breed were given either 250,000 (eight lambs in experiment 1) or 100,000 (six lambs in experiment 2) sporulated oocysts of Eimeria ovinoidalis (> 99.9% pure) via a stomach tube. Nine lambs (five in experiment 1 and four in experiment 2) of the same age served as uninoculated controls. The infected lambs developed diarrhea and dehydration between days 10 and 13 after infection. Intestinal specimens were collected 10-20 days after inoculation. A heavy multifocal infection of the epithelium comprising schizonts, gamonts, and oocysts resulted, with changes being most pronounced in the large intestine and the terminal part of ileum. Lesions included villous atrophy, villous fusions, and crypt hyperplasia. Computer-assisted morphometric analysis was applied to immunoperoxidase-stained sections for quantification of intraepithelial T cell subsets and included the distal jejunum of lambs of experiment 1. A significant increase in the epithelial area of the distal jejunum occupied by CD8+ lymphocytes was found in the infected lambs as compared with uninfected controls (18.3% versus 3.7%). The increased presence of CD8+ cells may be of significance in the intestinal immune response to ovine coccidiosis. A moderate, overall increase was also found for the gamma delta lymphocytes in the jejunal epithelium. Examination of the ileum of infected lambs of both experiments showed a scarcity of gamma delta cells (0.4% of epithelial area) in stretches of epithelium infected by coccida as compared with adjacent noninfected epithelium (4.0% of epithelial area).(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0300-9858
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
504-12
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Scarcity of gamma delta T cells in intestinal epithelia containing coccidia despite general increase of epithelial lymphocytes.
pubmed:affiliation
Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo, Norway.
pubmed:publicationType
Journal Article