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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1996-3-13
|
| pubmed:abstractText |
We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals were visualized by exposition to X-ray films and dipping autoradiography. The rank order of abundance was Gi alpha-2 approximately Gs alpha >> Go alpha. In general, G protein alpha-subunit mRNAs were evenly distributed in the heart including endo- and epicardium, large vessels and valves. Go alpha-mRNA levels were significantly higher in atria than in ventricles. In contrast to the rather uniform labeling of working myocardium, expression of all three G proteins was enriched in small intramural blood vessels and in subendocardial Purkinje fibers of septum and papillary muscles. A more marked enrichment of Gs alpha-, Gi alpha-2- and especially Go alpha-mRNA was seen in neuronal ganglionic cells in the atrial septum and posterior regions of the atrium. The main finding, however, was an enrichment of all three G protein mRNAs in the atrioventricular conductive tissue. The accumulation was strictly co-localized with acetylcholinesterase-positive regions identified as the atrioventricular node, the bundle of His and the right and left bundle branches and was seen similarly in rat and guinea-pig hearts. Quantitative in situ hybridization revealed Gs alpha-, Gi alpha-2- and Go alpha-mRNA levels in the bundle of His to be 206 +/- 0.13%. 191 +/- 0.15% and 165 +/- 0.06%, respectively, of that in the surrounding interventricular working myocardium. These findings indicate that heterotrimeric G proteins play an important role in modulation of electrical conductance in the heart.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-2828
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2249-63
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8576940-Acetylcholinesterase,
pubmed-meshheading:8576940-Adipose Tissue,
pubmed-meshheading:8576940-Animals,
pubmed-meshheading:8576940-Atrioventricular Node,
pubmed-meshheading:8576940-Brain Chemistry,
pubmed-meshheading:8576940-Female,
pubmed-meshheading:8576940-GTP-Binding Proteins,
pubmed-meshheading:8576940-Guinea Pigs,
pubmed-meshheading:8576940-Heart Septum,
pubmed-meshheading:8576940-Heart Ventricles,
pubmed-meshheading:8576940-In Situ Hybridization,
pubmed-meshheading:8576940-Lung,
pubmed-meshheading:8576940-Male,
pubmed-meshheading:8576940-Nerve Tissue Proteins,
pubmed-meshheading:8576940-Organ Specificity,
pubmed-meshheading:8576940-RNA, Messenger,
pubmed-meshheading:8576940-Rats,
pubmed-meshheading:8576940-Rats, Wistar
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.
|
| pubmed:affiliation |
Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus, Hamburg, Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|