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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1996-3-13
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pubmed:abstractText |
The structures of 3-isopropylmalate dehydrogenase (IPMDH) from Thermus thermophilus in complexes with its substrate, cofactor, and a cofactor analog were investigated by X-ray diffraction in a crystalline state and by small-angle X-ray scattering (SAXS) in solution. The structures at 2.8 A resolution of the complexes with the substrate, 3-isopropylmalate (IPM), and with an analog of NAD, ADP-ribose, were both very close to the structure of the free enzyme, which adopts an open conformation. However, the binding of a ligand induced a small conformational change near the binding site. This result contrasts with results for NADP(+)-bound and isocitrate-bound isocitrate dehydrogenase (ICDH) from Escherichia coli, which adopts a closed conformation. The SAXS analysis in solution clearly showed that IPMDH without a ligand adopts two distinct intermediate conformations, between the open and closed states, upon binding of NADH and IPM respectively, and adopts a fully closed conformation when in a ternary complex with NADH and IPM together.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
745-52
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:8576088-3-Isopropylmalate Dehydrogenase,
pubmed-meshheading:8576088-Alcohol Oxidoreductases,
pubmed-meshheading:8576088-Amino Acid Sequence,
pubmed-meshheading:8576088-Binding Sites,
pubmed-meshheading:8576088-Computer Simulation,
pubmed-meshheading:8576088-Crystallography, X-Ray,
pubmed-meshheading:8576088-Ligands,
pubmed-meshheading:8576088-Molecular Sequence Data,
pubmed-meshheading:8576088-Protein Conformation,
pubmed-meshheading:8576088-Sequence Alignment,
pubmed-meshheading:8576088-Thermus thermophilus
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pubmed:year |
1995
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pubmed:articleTitle |
Ligand-induced changes in the conformation of 3-isopropylmalate dehydrogenase from Thermus thermophilus.
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pubmed:affiliation |
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama.
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pubmed:publicationType |
Journal Article
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