Linked Life Data

8575763

Source:http://linkedlifedata.com/resource/pubmed/id/8575763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-3-14
pubmed:databankReference
pubmed:abstractText
The MEF2 genes belong to the MADS box family of transcription factors and encode proteins that bind as homo- and heterodimers to a consensus CTA(T/A)4TAG/A sequence, which is present in the regulatory regions of numerous muscle-specific and growth-inducible genes. Sequence analysis of human MEF2 cDNA clones suggests that they arose from alternatively spliced transcripts of four different genes, termed MEF2A-D. We have mapped the MEF2 genes to human chromosomal regions by identifying unique sequences in the MEF2 cDNA clones and using these sequences as PCR primers on the DNA of human-rodent hybrid clone panels that are informative for different regions of the human genome. PCR primers were also used to identify individual YAC clones for two of the genes, MEF2A and MEF2C, and a PCR product was used to identify cosmid clones for MEF2B. Genetic and physical mapping information available from genome databases on markers contained within YAC and cosmid clones provided independent assignments for those genes. Inter-Alu PCR painting probes of YAC clones were used as probes for high-resolution chromosomal regional assignment by fluorescence in situ hybridization. The localization of MEF2A to chromosome 15q26, MEF2B to 19p12, MEF2C to 5q14, and MEF2D to 1q12-q23 verifies the existence of at least four distinct loci for members of this gene family.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
704-11
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8575763-Alternative Splicing, pubmed-meshheading:8575763-Animals, pubmed-meshheading:8575763-Base Sequence, pubmed-meshheading:8575763-Chromosome Mapping, pubmed-meshheading:8575763-Chromosomes, Human, Pair 1, pubmed-meshheading:8575763-Chromosomes, Human, Pair 15, pubmed-meshheading:8575763-Chromosomes, Human, Pair 19, pubmed-meshheading:8575763-Chromosomes, Human, Pair 5, pubmed-meshheading:8575763-DNA Primers, pubmed-meshheading:8575763-DNA-Binding Proteins, pubmed-meshheading:8575763-Humans, pubmed-meshheading:8575763-Hybrid Cells, pubmed-meshheading:8575763-In Situ Hybridization, Fluorescence, pubmed-meshheading:8575763-MADS Domain Proteins, pubmed-meshheading:8575763-Molecular Sequence Data, pubmed-meshheading:8575763-Multigene Family, pubmed-meshheading:8575763-Myogenic Regulatory Factors, pubmed-meshheading:8575763-Polymerase Chain Reaction, pubmed-meshheading:8575763-Transcription, Genetic, pubmed-meshheading:8575763-Transcription Factors
pubmed:year
1995
pubmed:articleTitle
Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23.
pubmed:affiliation
Department of Medical Cell Biology, Alfred I. duPont Institute, Wilmington, Delaware 19899, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't