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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1996-3-12
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pubmed:abstractText |
A culture of Aspergillus nidulans (FGSC 359) was gradually adapted for growth in media containing up to 2 M NaCl or was exposed to a salt shock with 2 M NaCl. The intracellular glycerol level increased by about 7.9-fold in salt-adapted and 2.4-fold in salt-shocked cultures when compared to the unadapted culture. The biosynthetic pathway involved in the accumulation of glycerol was investigated under long-term salt adaptation and short-term salt shock. Glycerol-3-phosphate dehydrogenase (EC 1.1.1.8) was induced 1.4-fold in salt-shocked but not in salt-adapted cultures. An alternate enzymatic pathway involving glycerol dehydrogenase (NADP(+)-dependent) utilizing dihydroxyacetone (DHA) and/or DL-glyceraldehyde (DL-GAD) was induced by NaCl. DHA-dependent glycerol dehydrogenase activity was induced about 6.3-fold in salt-adapted and 1.35-fold in salt-shocked cultures, while DL-GAD-dependent activity was induced about 6.1-fold in salt-adapted and 1.2-fold in salt-shocked cultures. However, the level of glycerol dehydrogenase activity with DL-GAD as substrate was 7% of the DHA-dependent activity. We conclude that a salt-inducible NADP(+)-dependent glycerol dehydrogenase activity electrophoretically indistinguishable from previously described glycerol dehydrogenase I results in glycerol accumulation in salt-stressed A. nidulans.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerolphosphate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/NAD,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/Saline Solution, Hypertonic,
http://linkedlifedata.com/resource/pubmed/chemical/Sugar Alcohol Dehydrogenases,
http://linkedlifedata.com/resource/pubmed/chemical/glycerol dehydrogenase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0147-5975
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
241-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8574901-Aspergillus nidulans,
pubmed-meshheading:8574901-Glycerol,
pubmed-meshheading:8574901-Glycerolphosphate Dehydrogenase,
pubmed-meshheading:8574901-NAD,
pubmed-meshheading:8574901-NADP,
pubmed-meshheading:8574901-Oxidation-Reduction,
pubmed-meshheading:8574901-Saline Solution, Hypertonic,
pubmed-meshheading:8574901-Sugar Alcohol Dehydrogenases
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pubmed:year |
1995
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pubmed:articleTitle |
Biosynthetic pathways of glycerol accumulation under salt stress in Aspergillus nidulans.
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pubmed:affiliation |
Department of Botany and Microbiology, Auburn University, Alabama 36849-5407, USA.
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pubmed:publicationType |
Journal Article
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